ELEMENTS OF MINERALIZATION MECHANISM IN MANTLE DENTIN

牙本质的形成主要被认为是有机基质中的非胶原成分与矿物质相互作用的结果，这导致矿物质沉积在胶原支架上。虽然许多基质分子已被牵连在这个过程中，它们的存在的必要性和它们中的每一个对生物矿化的贡献程度仍然未知。其主要原因是存在明显不同的矿化机制，反映在牙本质中的有机基质的组成和性质以及形成矿物的性质在套髓牙本质和环髓牙本质区域之间的差异。高分辨率光谱分析技术与组织学相结合，优化了几乎所有有机和无机矿化元素的分析，为研究牙本质形成提供了一个很好的手段。由于套膜牙本质与环髓牙本质相比在有机基质和矿物性质上存在改变，因此，对套膜牙本质中牙本质形成的研究以及与环髓牙本质的比较有望进一步帮助建立矿化途径。为此，建议建立一个研究牛牙套膜牙本质形成的模型。通过将相同的方法应用于来自敲除特定基质大分子（目前为骨桥蛋白、骨唾液酸蛋白、骨连接素、双糖链蛋白聚糖和核心蛋白聚糖）的小鼠的牙本质，将评估每种大分子对于适当牙本质形成的必要性。

Dentin formation is largely viewed as the result of interaction of noncollagenous components of the organic matrix with mineral, which leads to the mineral deposition on a collagen scaffold. Although many matrix molecules have been implicated in this process, the necessity of their presence and extent of contribution of each of them to biomineralization remains unknown. The main reason for that is the apparent different rnineralization mechanisms that exist, reflected in dentin by the variation in the composition and properties of organic matrix and the properties of the formed mineral between areas of mantle dentin and circumpulpal dentin. The combination of a high-resolution spectroscopic analytical technique optimized for the analysis of virtually all organic and inorganic mineralization elements with histology provides an excellent means of studying dentin formation. As mantle dentin presents alterations in organic matrix and mineral properties compared to circumpulpal dentin, study of dentin formation in it and comparison to circumpulpal dentin is expected to further help establish the mineralization pathways. For this purpose, the development of a model for study of mantle dentin formation on bovine teeth is suggested. By applying the same methods to dentin from mice that are knockout for a specific matrix macromolecule (at the present time osteopontin, bone sialoprotein, osteonectin, biglycan and decorin) the necessity of each macromolecule for proper dentin formation will be assessed.