MEIOTIC GENETICS IN D DISCOIDEUM

唯一的目标是开发一个减数分裂遗传分析系统， 盘状网柄藓 网骨藻作为一个模式系统 用于研究单细胞变形虫运动和趋化性， 细胞命运的选择和调节。 主要的限制是 对这种单倍体生物体进行遗传分析的困难。 近年来，由于 同源基因置换研究多种细胞功能。 在野外，网囊藻有一个有性的二倍体周期。 不幸的是， 网囊藻的实验室菌株已经失去了这种能力。 凯辛 提出了建立网骨藻有性品系的方法， 对于基因作图、菌株的同源化和创造 多个变异种群

The single aim is to develop a system of meiotic genetic analysis for Dictyostelium discoideum. Dictyostelium has served as a model system for studying single cell amoeboid motility and chemotaxis and multi- cellular fate selection and regulation. The major limitation has been the difficulty in genetic analysis of this haploid organism. Dictyostelium has enjoyed a resurgence in recent years due the ease of homologous gene replacement to study a variety of cellular functions. In the wild, Dictyostelium has a sexual, diploid cycle. Unfortunately, the laboratory strains of Dictyostelium have lost this ability. Kessin proposes methods to establish sexual strains of Dictyostelium which will be essential for genetic mapping, isogenization of strains, and creating multiple mutant stocks.