ARTIFICIAL BREEDING OF CHIMPANZEES

这项计划是针对辅助育种的发展而制定的 黑猩猩和其他非人类灵长类动物的技术。方法 已经建立了精液收集和冷冻保存的方法 保存的精子的可育性得到证实。人的体外受精 黑猩猩的卵母细胞是常规的，已经没有足月怀孕了 黑猩猩在试管受精后，尽管我们已经确定了三种化学物质 怀孕。然而，我们相信我们已经确定了一个潜在的 造成这种情况的原因，这与 用于胚胎再植入的月经周期。因此，我们 将人工授精成功率的增加与 月经周期的内分泌变化使得在最佳周期中，Al 一次受精的成功率超过80%，而不是 未选择的周期约为20%。这一变化被假设为 关系到子宫内膜的发育和植入的成功。AS 由于目前暂停繁育绵羊，我们有 8-16细胞阶段的冷冻黑猩猩胚胎供未来使用 而不是立即使用它们进行再移植。资助美国国立卫生研究院/ RR03587-1151$207,223 4/01/93-8/31/98出版物古尔德，K.G.和 尤尼斯，A.I鱼抗冻蛋白在灵长类精子中的潜在应用 超低温保存。低温生物学(正在出版中)。尤尼斯，人工智能，鲁克斯，B 抗冻肽III(AFP)的作用 转铁蛋白硒(ITS)在胰岛素超低温保存中的应用 黑猩猩(黑猩猩)精子。J·安德鲁。19：207-214， 1998年。P51RR00165-38 1998年1月1日-1998年12月31日耶尔克斯地区灵长类 研究中心

This program has been directed to development of assisted breeding techniques for the chimpanzee and other nonhuman primates. Methods for collection and cryostorage of semen have been established and fertility of stored sperm demonstrated. In vitro fertilization of chimpanzee oocytes is routine, there has been no term pregnancy in the chimpanzee after IVF, although we have identified three chemical pregnancies. However, we believe we have identified a potential reason for this situation, which has to do with the characteristics of the menstrual cycle used for reimplantation of the embryos. Thus, we have correlated an increase in Al success with the pattern of the menstrual cycle endocrine changes such that in the optimum cycle, Al success rate is over 80% for a single insemination, as opposed to around 20% for unselected cycles. That change is hypothesized to relate to endometrial development and the success of implantation. As a result of the present moratoriu m on chim panzee breeding, we have cryobanked chimpanzee embryos at the 8 - 16 cell stage for future use rather than immediately using them for reimplantation. FUNDING NIH / RR03587-1151 $207,223 4/01/93 - 8/31/98 PUBLICATIONS Gould, K.G. and Younis, A.I Potential use of fish antifreeze proteins in primate sperm cryopreservation. Cryobiology (In press). Younis, A.I., Rooks, B., Khan, S. and Gould, K.G. The effects of antifreeze peptide III (AFP) in insulin transferrin selenium (ITS) on cryopreservation of chimpanzee (Pan troglodytes) spermatozoa. J. Androl. 19:207-214, 1998. P51RR00165-38 1/1/98 - 12/31/98 Yerkes Regional Primate Research Center