HORMONAL MANIPULATION OF MENSTRUAL CYCLES IN MACAQUES

这是对其他调查人员服务的延续， 提供已知胎龄的妊娠，并提供 恒河猴（Macaca mulatta）的同步月经周期。 为 定时繁殖猴子 周期是由日常控制的 注射孕酮（5 mg/天）10天。 雌性是成对的 在最后一次注射孕酮后第14至21天对雄性进行。 排卵和受孕发生在孕激素注射后的第16至18天。 注射剂 成功率，基于妊娠21天的存活胎儿， 在第一轮交配中保持在60%到70%。 胎儿发育是 用超声波监测。 用于月经同步 猴子每天检查两次以检测月经。 16日 周期的天数每日孕酮注射开始。 注射 直到所有要同步的猴子都收到了 至少注射10次。 此时，所有注射停止。 月经 发生在最后一次黄体酮注射后的第1或2天。 如果 研究者希望治疗处于黄体期的猴子， 在周期中，孕酮注射可以在第16 - 18天再次开始开始， 可将猴维持在黄体期用于研究。 基金基础赠款

This is a continuation of services to other investigators to provide pregnancies of known gestational ages and to provide synchronized menstrual cycles in rhesus monkeys (Macaca mulatta). For timed breeding, the monkeys? cycles are controlled by the daily injection of progesterone (5 mg/day) for 10 days. Females are paired with males on days 14 to 21 after the last progesterone injection. Ovulation and conception occurs on day 16 to 18 after the progesterone injections. Success rate, based on viable fetus at 21 days gestation, remains at 60% to 70% for first cycle matings. Fetal development is monitored using ultrasonography. For synchronization of menstrual cycles monkeys are examined twice daily to detect menses. On the 16th days of the cycle daily progesterone injections begin. Injections are continued until all the monkeys to be synchronized have received a minimum of 10 injections. At that point all injections cease. Menses occurs on day 1 or 2 following the last progesterone inj ection. If the investigator wishes to treat the monkeys in the luteal stage of the cycle, progesterone injections can begin again on day 16 - 18 and the monkeys can be maintained in a luteal stage for the study. FUNDING Base Grant PUBLICATIONS None