LUNG MATURATION

这个关于肺成熟的研究项目将集中在以下几个方面 提供动物肺成熟的功能和生化标记物 早产的Lamb Core对这个项目的贡献。这些动物将会是 以单独给药的方式进行超声波注射治疗胎儿 剂量和AS组合(皮质类固醇、T3、TRH和EGF)与之前 未经操控的胎羊。这个项目的目标是将 评估出生后肺功能对肺反应的影响 通过连续测量气体交换、肺顺应性、肺 容量(功能残气量和40 cmH2O时的肺容量 压力)和对外源表面活性物质的响应 早产后几个小时。随后，肺泡表面活性物质 饱和磷脂酰胆碱和SP-A池大小将测量为 抗氧化酶水平和SP-A、SP-B、SP-C的mRNA水平 在肺组织和从组织分离的II型细胞中。这些 多个终端将同时反映功能和生化 早产羔羊肺成熟诱导反应的评价 接受短期、临床相关的激素治疗 战略。

This Research Project on Lung Maturation will focus on a number of functional and biochemical markers of lung maturation in animals provided to this project by the Preterm Lamb Core. These animals will have been treated by ultrasound injections as fetuses with hormones given as single doses and as combinations (corticosteroid, T3, TRH and EGF) to previously unmanipulated fetal sheep. The goals of this project are to characterize the pulmonary responses by evaluating postnatal lung function as assessed by sequential measurements of gas exchange, lung compliances, lung volumes (functional residual capacity and lung volumes at 40 cmH2O pressures) and responses to exogenous surfactant over the first five hours after preterm delivery. Subsequently, alveolar surfactant saturated phosphatidylcholine and SP-A pool sizes will be measured as will antioxidant enzyme levels and SP-A, SP-B, and SP-C in mRNA levels in lung tissue and type II cells isolated from the tissue. These multiple endpoints will reflect both functional and biochemical assessments of the induced lung maturation responses in preterm lambs exposed to short duration, clinically relevant hormone treatment strategies.