OLD YELLOW ENZYME--CLONING AND STRUCTURE

我们一直在使用由General Clinical维护的GCG包 研究中心在研究老黄酶（OYE）。 三种同工酶 酵母OYE基因的克隆和序列测定。 这些 序列具有高度同源性，但OYE的生理功能 仍然未知。 我们继续寻找具有序列的酶 同源性以试图鉴定功能。 的晶体结构 OYE的一种同工酶已经被解决了， 确定对配体结合和NADPH影响的重要残基 酶的氧化。 我们计划使用 GCG程序设计用于额外突变的引物。 总 实验室的重点是研究含核黄素的酶。 我们 目前正在克隆和鉴定其中的两个， 酶、D-氨基酸氧化酶和2-甲基-3-羟基吡啶-5-基 羧酸加氧酶。 在这些研究中，我们将继续使用 GCG包的特点。

We have been using the GCG package maintained by the General Clinical Research Center in the study of Old Yellow Enzyme (OYE). Three isozymes of OYE from Saccharomyces have been cloned and sequenced. These sequences have a high homology, but the physiological function of OYE is still unknown. We continue to look for enzymes with sequence homology in an attempt to identify function. The crystal structure of one of the isozymes of OYE has been solved and we have been mutating significant residues to determine the effect on ligand binding and NADPH oxidation by the enzyme. We plan to use several of the features of the GCG program to design primers for additional mutation. The general focus of the lab is the study of riboflavin-containing enzymes. We are currently working on the cloning and characterization of two of these enzymes, D-Amino Acid Oxidase and 2-Methyl-3-Hydroxypyridine-5- Carboxylic Acid Oxygenase. In these studies we will continue to use the features of the GCG package.