CORE--CELL BIOLOGY AND PHYSIOLOGY

联合使用核心设施是运营中的一项关键活动 一项计划项目。这个细胞生物学和生理学核心带来了 4个项目的调查人员，以便利获得专门的 技术和更新的方法。 该核心的目标是提供：1)技术援助和 电生理学和分子生物学技术培训以及 调查人员和辅助人员的方法；2)中央 数据解释咨询服务；3)中央设施 对于包括离子通量研究的共聚焦显微镜(例如 Ca~(++)、H~+)和细胞骨架及膜蛋白 测定蛋白质合成、氨基酸掺入的组织 以及4)用于制造和使用膜片钳的设施， 细胞内外离子的离子选择微电极 测量。 我们相信，这一核心将促进调查人员之间的互动 一个项目的结果可能会影响后续的设计和 程序中的其他协议，正如我们在总体上详细描述的那样 以上是本节目的介绍。

Joint use of Core facilities is a crucial activity in the operation of a Program Project. This Cell Biology and Physiology Core brings in investigators of 4 projects to facilitate access to specialized technologies and newer methods. The aims of this Core are to provide: 1) technical assistance and training in electrophysiologic and molecular biologic techniques and approaches to investigators and supporting staff; 2) central consultative service for interpretation of data; 3) centralized facility for confocal microscopy which includes the study of ionic fluxes (e.g. Ca++, H+) and cytoskeletal and membrane proteins in live and fixed tissues for measurements of protein synthesis, amino-acid incorporation etc. and 4) a facility for fabrication and utilization of patch clamp, ion-selective microelectrodes for intracellular and extracellular ionic measurements. We believe that this Core will foster interactions between investigators and results from one project may affect the subsequent design and protocols in others within the program as we detailed in the overall introduction of this program above.