DEVELOPMENT OF AN OPTICAL WORKSTATION

我们正在开发一种光学工作站，它将允许 同时进行多光子成像和多光子去噪。这个 工作站将配备两个超快、短脉冲、全固态 促进三维多光子激发的激光 活细胞和组织的局部去化和成像。这个 UNCAMING激光器将是由 Microlase有限公司将以120阿秒的速度产生120个费秒脉冲 NfHZ波长为650 nm，平均功率为50毫瓦。去中心化 图案(体积)将由用户通过软件定义。成像 激光是一种可调谐的光谱物理尼菲尔尼亚/海啸费秒 带有中镜的钛蓝宝石激光器，输出功率为60 从770 nm到910 nm的80 MHz频率的飞秒脉冲，平均 功率&GT；500兆瓦整个范围。外脉冲补偿 将被实施来将这些超短脉冲保持在 样本。激光扫描将通过NMC 1024硬件和 软件。每台激光都有自己独立的扫描系统。这个 扫描的输出将被合并并引导到长时间的工作中 安装在尼康量子望远镜上的距离目镜扩束器 倒置显微镜。直接检测将通过 量子凯勒洞。蓝色、绿色和红色发射荧光团 该系统可以使用400 nm到640 nm的范围。这个 显微镜将被放置在一个三气培养箱内，以 促进活细胞成像作为激发的函数 波长。

We are developing an optical workstation that will allow simultaneous multiphoton imaging and multiphoton uncaging. The workstation will have two ultra-fast, short-pulse, all-solid-state lasers to facilitate multiphoton excitation for 3-dimensionally localized uncaging and imaging of living cells and tissues. The uncaging laser will be a prototype Nd:YLF laser manufactured by Microlase, Ltd. which will produce 120 ferntosecond pulses at a 120 NfHZ rate at 650 nm with a mean power of >50 mwatts. The uncaging pattern (volume) will be user definable via software. The imaging laser is a tunable Spectra Physics Nfillenia/ Tsunami ferntosecond titanium sapphire laser with a mid mirror set which produces 60 ferntosecond pulses at a 80 MHz rate from 770 nm to 910 nm with a mean power >500 mwatts throughout the range. External pulse compensation will be implemented to maintain these ultra-short pulses at the sample. Laser beam scanning will be handled via NMC 1024 hardware and software. Each laser has its own independent scanning system. The scanned outputs will be combined and directed into a long working distance eyepiece beam expander attached to a Nikon Diaphot Quantum inverted microscope. Direct detection will be implemented via the Quantum Keller hole. Blue, green, and red emitting fluorophores in the range from 400 nm to 640 nm may be used with the system. The microscope will be contained within a three-gas incubator to facilitate live cell imaging viability as a function of excitation wavelength.