MACINTOSH SOFTWARE DVMT FOR VIEWING & ANNOTATION OF 4D LIVE CELL DATA SETS

概述3D时间推移(41)采集可以产生数据集 其中包括数千到数万个单独的图像。在……里面 为了处理这些数据中包含的大量图像 集合，显然需要一种简单的方法来 在时间上向前和向后导航，在焦点上上下导航 对样本进行平整。此任务由4D查看器处理 申请。设计为编写的独立Macintosh应用程序 在C语言中，4D查看器使4D导航任务变得很容易。 在加载由4D产生的QuickTime数据集之后 周转应用程序或4D Grabber软件，用户可以使用 鼠标或键盘可以向后或向前播放电影，切换 事件以流畅的动画形式展开。用户可以 同时从样品的顶部到底部咆哮， 在关注的事件之后。书签可以放置在不同的位置 4D空间中的位置提供对特定活动的即时访问，以及 可以将这些书签保存到磁盘上，以供后续数据分析 会话。“转到”功能将允许用户跳转到任何 他们想要的时间点和焦平面，以及“您在这里”功能 将告诉用户他们在数据集中的当前位置。电影可以 被循环，以选择的速度玩，在任何一种情况下玩 方向，或者更仔细地一次检查一个名字。在……里面 除了浏览和查看数千个 对于组成数据集的图像，4D查看器还有其他强大的工具 在它的武器库里。编辑套件允许用户创建颜色 可突出显示图像中感兴趣的要素的覆盖。 圆、正方形、手绘和直线、文本和箭头，全部在 可使用各种颜色和大小来注记存在的要素 在图像中。通过在后续帧中操纵这些对象， 可以创建跟随移动要素的动态彩色叠加 并保存到磁盘上。因为这些叠加层被放在图像上 当电影正在播放，并且可以打开或关闭时，他们会这样做 不会影响它们下面的图像信息。另外， 因为它们是基于指令而不是基于位图的对象， 它们只需占用很少的磁盘空间即可存储。5000个中等大小 覆盖只能存储在9Mb的磁盘空间中。加强这一点 非常强大的批注工具是关联隐藏文本的能力 长方体，每个对象都在一个覆盖中。通过在圆圈上单击鼠标 来突出显示特定的单元格，例如，文本框可以是 它可能勾勒出细胞的位置、命运和名称。一 可以很容易地看到一个经过充分注释和文本增强的数据集， 放在带有4D查看器应用程序的CD-ROM上，将是一个 令人难以置信的强大工具，无论是用于传递研究成果，还是 用于教育目的。4D Live Cell的各种硬件组件 显微镜工作站，但使用易于理解的图形 用户界面，以减少 希望自己进行这些类型的实验的研究人员 实验室。该软件支持6种常见的数字化帧采集卡 Nubus和PCI插槽配置(包括以下功能 利用卡上帧平均(如果存在)，并支持 四个不同的阶段驱动电机。它的模块化代码设计使 添加对其他硬件系统的支持非常简单。 还实现了收集速度的显著提高 软件的优化设计。“4D”的另一个优势 Grabber“软件是将4D数据直接收集到 压缩的QuickTime电影。通过利用此功能，它可以 可以收集多达15倍的数据点 硬盘空间比使用以前的4D采集系统时更大。这 收藏模式还提供了非常可观的节省

OVERVIEW A 3D time-lapse (41)) acquisition can result in data sets which include thousands to tens of thousands of individual images. In order to deal with the sheer volume of images contained in these data sets, it was clear that a straightforward method would be needed to navigate forward and backward in time as well as up and down in focal level through the sample. This task is handled by the 4D Viewer application. Designed as a stand-alone Macintosh application written in C, the 4D Viewer makes the task of 4D navigation an easy one. After loading a Quicktime data set that was produced either by the 4D Turnaround application or the 4D Grabber software, the user can use the mouse or keyboard to play the movies backward or forward, witching events unfold as smoothly flowing animations. The user can simultaneously roarn from the top to the bottom of the sample, following events of interest. Bookmarks can be placed at various locations in 4D space providing instant access to specific events, and these bookmarks can be saved to the disk for subsequent data analysis sessions. A "Go To" function will allow the user to jump to any timepoint and focal plane they wish, and a "You are Here" function will tell the user their current location in the data set. Movies can be looped, played at a selection of speeds, played in either direction, or examined more carefully one ftame at a time. In addition to the basic task of moving through and viewing the thousands of images making up a data set, the 4D Viewer has other powerful tools in its arsenal. An editing suite allows the user to create color overlays which can highlight features of interest the in the image. Circles, squares, freehand and straight lines, text and arrows, all in a variety of colors and sizes can be used to annotate features present in the image. By manipulating these objects in subsequent frames, dynamic, color overlays which follow moving features can be created and saved to the disk. Because these overlays are put onto the image as the movie is being played and can be switched on or off, they do not affect the image information that is under them. Additionally, because they are instiuction-based rather than bitmap-based objects, they take very little disk space to store. 5000 average-sized overlays can be stored in only 9 Mb of disk space. Enhancing this very powerful annotation tool is the ability to associate hidden text boxes with each object in an overlay. By clicking a mouse on a circle that highlights a specific cell, for example, a text box can be brought up which may outline the cell's position, fate and name. One can easily see how a fully annotated and text-enhanced data set, placed onto a CD-ROM with he 4D Viewer application, would be an incredibly powerful tool either for passing on research results, or for educational purposes.various hardware components of a 4D Live Cell microscopy workstation, but does so using easy to-understand graphic user interfaces in order to reduce the learning curve for the researcher wishing to perform these types of experiments in their own labs. The software supports 6 common digitizing framegrabber cards of both the Nubus and PCI slot configuration (including the ability to take advantage of on-card frame averaging, if present), and supports four different stage drive motors. Its modular code design makes adding support for additional hardware systems very straightforward. Significant increases in collection speed were also realized with the software's optimized design. An additional advantage of the "4D Grabber" software is the ability to gather 4D data directly to compressed QuickTime movies. By taking advantage of this feature, it is possible to gather up to 15 times as many data points per given hard disk space than when using previous 4D collection systems. This collection mode also offers a very impressive savings