COMPARISON OF I & 2 PHOTON IMAGING OF KIDNEY TISSUE

为了准备一笔赠款，以资助一台位于 印第安纳大学我们想在杜氏大学使用这一系统 获取初步数据。我们会把固定的Iddney组织样本 它已经被罗丹明荧光团染色了。弗罗利希博士会 用多光子YLF固体激光器观察样品 成像和ADLAS 532运行固态激光进行共焦成像。 我们希望她收集这种固定材料成像的数据集 首先通过共聚焦显微镜，然后通过 多光子显微镜。ZSeries和xzSeries数据集将是 收集以记录成像穿透深度的改进 多光子显微镜与共聚焦获得的结果比较 显微镜。数据会通过匿名FI传输给我们吗？ 网站由先生负责维护。

In preparation for a grant to fund a multiphoton microscope at the University of Indiana we would like to use the system at the MR to obtain preliminary data. We will send fixed samples of Iddney tissue which has been stained with rhodamine fluorophores. Dr. Frohlich will observe the samples using the YLF solid-state laser for multiphoton imaging and the ADLAS 532 run solid state laser for confocal imaging. We would like her to collect data sets of this fixed material imaged first by confocal microscopy and then sample the same volume by multiphoton microscopy. Zseries and xzseries data sets will be collected to document the improvement in depth of imaging penetration obtained with multiphoton microscopy compared with confocal microscopy. The data will be transmitted us me via the anonymous FI? site maintained by the MR.