OLD YELLOW ENZYME--CLONING AND STRUCTURE

我们一直在使用由综合临床中心维护的GCG包 老黄酶(OYE)研究中心。三种同工酶 已从酵母菌中克隆并测序了OYE。这些 序列同源性很高，但OYE的生理功能 仍然是未知的。我们继续寻找有序列的酶 同源性试图识别功能。该化合物的晶体结构 Oye的一种同工酶已经被解决，我们一直在突变 确定有效残基对配体结合和NADPH的影响 酶的氧化作用。我们计划使用tthe的几个功能 GCG程序为额外的突变设计引物。这位将军 该实验室的重点是研究含有核黄素的酶。我们是 目前正在克隆和鉴定其中的两个 酶，D-氨基酸氧化酶和2-甲基-3-羟基吡啶-5- 羧酸加氧酶。在这些研究中，我们将继续使用 GCG包的功能。

We have been using the GCG package maintained by the General Clinical Research Center in the study of Old Yellow Enzyme (OYE). Three isozymes of OYE from Saccharomyces have been cloned and sequenced. These sequences have a high homology, but the physiological function of OYE is still unknown. We continue to look for enzymes with sequence homology in an attempt to identify function. The crystal structure of one of the isozymes of OYE has been solved and we have been mutating significant residues to determine the effect on ligand binding and NADPH oxidation by the enzyme. We plan to use several of the features of tthe GCG program to design primers for additional mutation. The general focus of the lab is the study of riboflavin-containing enzymes. We are currently working on the cloning and characterization of two of these enzymes, D-Amino Acid Oxidase and 2-Methyl-3-Hydroxypyridine-5- Carboxylic Acid Oxygenase. In these studies we will continue to use the features of the GCG package.