ADRENAL CHROMAFFIN CELL NA+ CHANNELS

这些被提议的研究的目标是研究分子基础。 肾上腺嗜铬细胞的神经分泌，特别强调 关于电压敏感性钠离子通道的作用。具体目标 这一建议包括确定分子结构 用分子克隆技术研究肾上腺嗜铬细胞Na+通道 这些Na+通道在非洲爪哇卵母细胞和哺乳动物细胞中的表达 评估它们的生理和药理特性，以及 最后，切除肾上腺嗜铬细胞中选定的Na+通道基因 用反义mRNA技术检测原代培养的细胞 儿茶酚胺神经分泌的哪些方面是由 特定的Na+通道亚基。我假设是因为钠离子通道 在其他组织中由辅助β亚基功能调节， 肾上腺嗜铬细胞的Na+通道β亚基将调节 神经内分泌Na+通道，因此使显著 对神经分泌调节的贡献。

The goal of these proposed studies is to investigate the molecular basis of neurosecretion in adrenal chromaffin cells, with particular emphasis on the role played by voltage-sensitive Na+ channels. The specific aims of this proposal include determination of the molecular structure of adrenal chromaffin cell Na+ channels by molecular cloning techniques, expression of those Na+ channels in Xenopus oocytes and mammalian cells to assess their physiological and pharmacological properties, and finally, ablation of selected Na+ channel genes in adrenal chromaffin cells in primary culture using antisense mRNA technology to determine which aspects of catecholamine neurosecretion are contributed by specific Na+ channel subunits. I hypothesize that because Na+ channels in other tissues are functionally modulated by auxiliary beta subunits, Na+ channel beta subunits in adrenal chromaffin cells will modulate neuroendocrine Na+ channels and therefore make a significant contribution to the regulation of neurosecretion.