OBSERVE CHANGES IN CELLULAR AUTOFLUORESCENCE DURING EARLY STAGES OF APOPTOSIS

在过去的一年里，细胞色素c从线粒体释放 已被确认为细胞凋亡过程中的早期事件，或者 程序性细胞死亡。此外，线粒体膜的丢失 在线粒体通透性的过程中观察到了电位 过渡，这是由氧化应激损伤引起的。这有 导致我们询问线粒体NADH的多光子成像 可能提供了一种观察线粒体功能障碍的技术 外源线粒体探针的使用。我们最近的努力已经 一直致力于建立诱导细胞凋亡和/或MPT的方案 在啮齿动物和/或人类癌细胞系中。未来的实验将 试图将线粒体自发荧光的变化与 线粒体形态和膜电位的观察 荧光探头。

Within the last year, cytochrome c release from the mitochondria has been identified as a very early event during apoptosis, or programmed cell death. Furthermore, a loss of mitochondrial membrane potential is observed during the mitochondrial permeability transition, which results from oxidative stress injuries. This has led us to inquire whether multiphoton imaging of mitochondrial NADH may provide a technique to observe mitochondrial dysfunction without the use of exogenous mitochondrial probes. Our recent efforts have been directed at establishing protocols to induce apoptosis and/or mPT in rodent and/or human cancer cell lines. Future experiments will seek to correlate changes in mitochondrial autofluorescence with mitochondrial morphology and membrane potential as observed with fluorescent probes.