CHEM & BIOL OF DNA ADDUCT: ANALYSIS OF DNA ADDUCTS HAVING MULTIPLE CONFORMATIONS

构象异质性正在成为一个共同的特点， 与化学致癌物加合的寡脱氧核苷酸。 光谱学上，构象异质性引入了几个 问题 首先，每个构象产生的共振信号 样品中存在的所有物质必须单独分离和鉴定。 第二，构象异质性降低了有效样本 浓度. 第三，结构优化的方法， 加合物不发达。 超高场NMR提供了一种 检测构象不稳定样品的有吸引力的方法 因为它具有非凡的分辨率和灵敏度， 识别和分配由构象交换产生的共振。 我们建议利用这一点， 苯乙烯氧化物所涉及的构象平衡， 腺嘌呤的苯并[a]芘加合物 结构与功能

Conformational heterogeneity is emerging as a common feature of oligodeoxynucleotides adducted with chemical carcinogens. Spectroscopically, conformational heterogeneity introduces several problems. First, resonance signals arising from each conformation present in the sample must be individually resolved and identified. Second, conformational heterogeneity reduces the effective sample concentration. Third, methodology for structural refinement of these adducts is not well-developed. Ultra high field NMR provides an attractive method for examining conformationally labile samples because of its extraordinary resolving power and sensitivity, to help identify and assign resonances which arise from conformation exchange. We propose to take advantage of this to develop a better understanding of the conformational equilibria involved for styrene oxide and benzo[a]pyrene adducts of adenine, in an effort to correlate adduct structure with function.