BIOLOGICAL FUNCTIONS OF ENTACTIN

描述：细胞外基质在正常的 复杂有机体的功能。 它与器官形成、细胞 分化、转移、神经元引导、伤口愈合，以及 保护功能。 一种特殊的片状结构， 细胞外基质，即基底膜，位于各种组织之下， 与上面细胞的质膜紧密接触。 的 这种结构的主要成分是层粘连蛋白、IV型胶原蛋白、串珠素 和巢蛋白/巢蛋白。 该提案涉及的生物功能 作为基底膜的主要成分的巢蛋白。 埃森是个 由1217个氨基酸残基组成的硫酸化糖蛋白。 分子 被组织成结构和功能域， 与层粘连蛋白、胶原IV、纤连蛋白、串珠蛋白聚糖、纤蛋白和 纤维蛋白原。 一个主要结合点被认为是组织 和基底膜的稳定。 此外，entactin和 来自巢蛋白的肽增强了 几种类型的细胞和促进双方的趋化性和吞噬作用， 中性粒细胞 巢蛋白基因的失活导致胚胎 小鼠的致死表型。 该项目的目的是扩大 本文综述了巢蛋白在小鼠胚胎发生、器官发育、 发育和维持组织功能。 在这些研究中 在巢蛋白基因的特定区域的突变将被引入到 小鼠胚胎干细胞移植 这些努力将 并辅以实验， 特异性组织的cre重组酶/loxP系统。 胚胎和 将分析突变动物的组织以将突变与 与组织形态和功能的关系。 希望结果 将有助于我们理解基底膜功能， 在正常发育和病理条件下的分子水平。

DESCRIPTION: The extracellular matrix plays a vital role in the normal function of complex organisms. It is implicated in organogenesis, cell differentiation, metastasis, neuronal guidance, wound healing, and protective functions. A specialized sheet-like structure of the extracellular matrix, the basement membrane, underlies various tissues and is in intimate contact with the plasma membrane of the overlying cells. The major components of this structure are laminin, type IV collagen, perlecan and entactin/nidogen. This proposal addresses the biological functions of entactin as a major component of the basement membrane. Entactin is a sulfated glycoprotein consisting of 1217 amino acid residues. The molecule is organized into structural and functional domains that are engaged in interactions with laminin, collagen IV, fibronectin, perlecan, fibulin and fibrinogen. A major junction of entactin is believed to be the organization and stabilization of the basement membrane. In addition, entactin and peptides derived from entactin enhance the attachment and spreading of several types of cells and promote both chemotaxis and phagocytosis in neutrophils. The inactivation of the entactin gene results in an embryonic lethal phenotype in the mouse. It is the purpose of this project to extend the studies on the role that entactin plays in mouse embryogenesis, organ development, and maintenance of tissue functions. In these studies mutations in specific regions of the entactin gene will be introduced into mice by means of modified embryonic stem cells. These efforts will be complemented with experiments to inactivate the gene for entactin in specific tissues by the cre recombinase/loxP system. The embryos and tissues of the mutant animals will be analyzed to correlate the mutations in entactin with tissue morphology and function. It is hoped that the results obtained will contribute to our understanding of basal lamina function at the molecular level in normal development and in pathological conditions.