NEIBANK: EST Analysis/Bioinformatics for Ocular Genomics

眼睛是一个复杂的系统，由各种发育起源的高度分化的组织组成。许多对眼睛功能至关重要的基因是组织特异性的，其中许多已知的基因与遗传性眼病有关。大多数人类表达的基因仅通过表达序列标签（EST）已知。然而，除视网膜外，许多眼组织在迄今为止有助于dbEST的cDNA文库中表现不佳。为了解决这种情况，已经启动了一个项目，NEIBANK，以改善人眼的EST覆盖范围。构建了透镜、睫状体/虹膜和RPE/脉络膜的cDNA文库。对来自每个未标准化文库的2000个克隆进行测序、分析和聚类。已经鉴定了几种新的基因，包括眼聚糖（一种眼特异性LRR蛋白，其基因图谱接近ARMD）、LGS（一种与谷氨酰胺合成酶相关的丰富的透镜特异性转录物）、眼斯潘（一种新的四斯潘，其图谱接近chr 17）等。正常化和更深层次的序列正在进行中。这一资源对于微阵列和全长cDNA收集将是有价值的。- 生物信息学，cDNA，基因组学

The eye is a complex system of highly differentiated tissues of various developmental origins. Many genes essential for eye function are tissue-specific and many of those known are associated with genetic eye diseases. The majority of human expressed genes are known only through expressed sequence tags (ESTs). However, with the exception of retina, many eye tissues have been poorly represented in the cDNA libraries that have so far contributed to dbEST. To address this situation a project, NEIBANK, has been initiated to improve the EST coverage of the human eye. cDNA libraries for lens, ciliary body/iris and RPE/choroid have been constructed. 2000 clones from each un-normalized library have been sequenced, analyzed and clustered. Several novel genes have been identified, including Oculoglycan (an eye-specific LRR protein whose gene maps close to ARMD), LGS (an abundant lens specific transcript related to glutamine synthestase), Oculospanin (a novel tetraspan that maps to chr17) and others. Normalization and deeper sequence is under way. This resource will be of value for micro-arrays and for full- length cDNA collections. - EST, Bioinformatics, cDNA, genomics