QUANTITATION OF VIRAL REPRODUCTION

没有可靠的通用方法来量化一个普通病毒复制的时间。从宏观上讲，一种病毒在一种培养物中的平均产仔数和繁殖时间是多少？该项目开发了数学和实验技术，用于确定单个母体病毒粒子附着在细胞上时的子体病毒粒子的平均数、子体的平均周期时间以及病毒周期时间的标准差(传播)。这些测定可以通过流式细胞仪实验进行。靶细胞用脂类染料染色，病毒通过与染色的第0代细胞孵育，在它们的贴壁阶段同步。通过收集感染标记物(在实际实验中，这将是HIV中的gp41)的数据，无论是在第0代染色细胞中还是在后来添加并与受感染的第0代细胞孵育的未染色细胞中，平均病毒的繁殖都可以作为积分方程式的解来确定。在将一群同类动物放生到野外并进行几次种群计数后，原则上可以使用相同的方法来计算一只动物的窝大小和繁殖周期。然而，在医学方面，这项工作的目的是改进各种艾滋病毒疗法的量化及其对艾滋病毒繁殖的抑制作用。计算机程序现在已经就位，一篇理论论文已经写好，实验方案正在实施中。-人类免疫缺陷病毒、数学建模、量化繁殖、病毒检测

There is no dependable general way of quantifying the times at which an average virus reproduces. In macroscopic terms, what are the litter sizes and breeding times of an average virus in a culture? This project develops mathematical and experimental techniques for determining the average number of daughter virions from a single mother virion, when these daughters attach to their cell, the average cycle time for the daughters, and the standard deviation (spread) of the viral cycle time. These determinations can be made from flow cytometry experiments. Target cells are stained with lipid dyes, and viruses syncronized at their attachment phase by incubating them with the stained generation 0 cells. By collecting data on a marker of infection (in actual experiments, this will be gp41 inHIV), both in generation 0 stained cells and unstained cells that are added later and incubated with the infected generation 0cells, the reproduction of the average virus can be determined as the solution of an integral equation. The same methods can in principle be employed in calculating litter sizes and breeding cycle times of an animal, after a homogeneous group of the animals is released into the wild and several population counts taken. In the medical context, however, this work is intended to improve the quantitation of various HIV therapies and their inhibitory effects on HIV reproduction. Computer programs are now in place, a theoretical paper has been written, and experimental protocols are now being carried out. - human immunodeficiency virus, mathematical modeling, quantifying reproduction, viral assays