HIV & TB INTERACTION IN LUNG

本研究主要研究HIV-1在肺泡巨噬细胞中复制的转录调控。来自非炎症肺的肺泡巨噬细胞通过表达16 kDa抑制形式的转录因子C/EBPB(也称为NF-IL6和LIP)来抑制HIV-1的复制，从而在肺部产生潜在的HIV-I感染。对结核病的炎症反应减少了C/EBPB的表达，降低了HIV-1的长端重复序列，并诱导了C/EBP转录因子家族的另一个成员，该转录因子家族支持HIV-1在肺部的高水平复制。该实验室用于以下方面：DNA提取、DNA测序(自动化)、ELISA、寡核苷酸合成、聚合酶链式反应、重组DNA技术、RNA提取、Southern分析、Western分析、支气管肺泡灌洗处理、EMSA和层流罩的使用。

This study focuses on the transcriptional regulation of HIV-1 replication in alveolar macrophages. Alveolar macrophages from uninflamed lung inhibit HIV-1 replication by expressing a 16kDa inhibitory form of transcription factor C/EBPB (also called NF-IL6 and LIP) producing latent HIV-I infection in the lung. The inflammatory response to tuberculosis reduces C/EBPB expression, derepressing the HIV-1 long terminal repeat and induces another member of the C/EBP transcription factor family which supports high level HIV-1 replication in the lung. The lab was used for the following: DNA isolation, DNA sequencing (automated), ELISA, oligonucleotide synthsis, PCR, recombinant DNA techniques, RNA isolation, Southern analysis, Western analysis, bronchoalveolar lavage processing, EMSA, and use of laminar flow hoods.