HIV/TB INTERACTION IN THE LUNG

HIV/结核病在肺部的相互作用

• 批准号: 7718381
• 负责人: Michael D. Weiden
• 金额: $ 0.1万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7718381
• 关键词: Alveolar Macrophages; Bronchoalveolar Lavage; CCAAT-Enhancer-Binding Protein-beta; CCAAT-Enhancer-Binding Proteins; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Sequence; EMSA; Enzyme-Linked Immunosorbent Assay; Family; Funding; Grant; HIV-1; Infection; Inflammatory Response; Institution; Lip structure; Long Terminal Repeats; Lung; Oligonucleotides; Polymerase Chain Reaction; Process; RNA; Recombinant DNA; Research; Research Personnel; Resources; Source; Techniques; Transcriptional Regulation; Tuberculosis; United States National Institutes of Health; factor C; member; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study focuses on the transcriptional regulation of HIV-1 replication in alveolar macrophages. Alveolar macrophages from uninflamed lung inhibit HIV-1 replication by expressing a 16kDa inhibitory form of transcription factor C/EBPB (also called NF-IL6 and LIP) producing latent HIV-I infection in the lung. The inflammatory response to tuberculosis reduces C/EBPB expression, derepressing the HIV-1 long terminal repeat and induces another member of the C/EBP transcription factor family which supports high level HIV-1 replication in the lung. The lab was used for the following: DNA isolation, DNA sequencing (automated), ELISA, oligonucleotide synthesis, PCR, recombinant DNA techniques, RNA isolation, Southern analysis, Western analysis, bronchoalveolar lavage processing, EMSA, and use of laminar flow hoods.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的重点是HIV-1在肺泡巨噬细胞中复制的转录调控。来自未发炎肺的肺泡巨噬细胞通过表达16 kDa抑制形式的转录因子C/EBPB（也称为NF-1 L 6和LIP）来抑制HIV-1复制，从而在肺中产生潜伏的HIV-1感染。对结核病的炎症反应降低了C/EBPB表达，解除了HIV-1长末端重复序列的抑制，并诱导了C/EBP转录因子家族的另一个成员，该转录因子家族支持HIV-1在肺中的高水平复制。该实验室用于以下方面：DNA分离、DNA测序（自动化）、ELISA、寡核苷酸合成、PCR、重组DNA技术、RNA分离、Southern分析、Western分析、支气管肺泡灌洗处理、EMSA和层流罩的使用。