PHASE I SINGLE DOSE TRIAL OF PEITC (PHENETHYL ISOTHIOCYANATE)

异硫氰酸苯乙酯(PEITC)是十字花科蔬菜中的一种天然化合物。已经在实验室和大鼠身上证明了它可以抑制香烟烟雾中的一种强致癌物质4-(methynitrosoamino)-1-(3-pyridyl)-1-butanone或Nnk。这项第一阶段研究将确定PEITC的最大耐受单次剂量；测量PEITC的pk以预测在NNK暴露的几个小时内维持PEITC稳定状态所需的每日剂量间隔；并获得关于PEITC对人体NKK代谢的干扰的初步数据。核心实验室用于聚合酶链式反应、酶联免疫吸附试验、层流罩和重组DNA技术。

Phenethyl Isothiocyanate (PEITC) is a naturally occuring compound found in cruciferous vegetables. It has been shown in the laboratory and in rats to inhibit 4-(methynitrosoamino)-1-(3-pyridyl)-1-butanone or NNK, a potent carcinogen in cigarette smoke. This Phase I study will determine the maximum tolerated single dose of PEITC; measure the PK of PEITC to predict the daily dosing interval required to maintain a steady state of PEITC in the hours of NNK exposure; and obtain preliminary data on the perturbation of NKK metabolism by PEITC in human beings. The Core Laboratory was utilized for PCR, ELISA, laminar flow hoods, and recombinant DNA techniques.