VITAMIN D RECEPTOR GENE POLYMORPHISM AND BREAST CANCER

实验证据和生态相关性研究支持 维生素D可能降低乳腺癌风险的假设。然而，这部小说 这一假设在很大程度上尚未在人类群体中得到验证。正在进行的 西班牙裔乳腺癌的人群病例对照研究， 非裔美国人和白色妇女将评估与 阳光照射和膳食维生素D摄入量。正在进行的分子 该应用的一项调查的研究表明， 维生素D受体（VDR）基因的多态性与维生素D受体（VDR）基因的多态性有关。 乳腺癌风险。因此，我们建议扩大正在进行的案件- 对照研究，以扩大我们的关系， 维生素D.除了正在收集的访谈数据外，我们建议 为一部分研究参与者采集血样， 10个月内完成病例和对照确定。符合条件的案件将 包括35-79岁被诊断患有浸润性乳腺癌妇女 1997年5月至1999年2月。个案将透过 大旧金山弗朗西斯科湾地区的癌症登记处;控制将 通过随机数字拨号从普通人群中选择。 专业的面试官将收集面试数据，测量皮肤 色素沉着，并通过家访获得血液标本。血液 样本和风险因素数据将可用于估计640 病例（245名西班牙裔，225名白人和170名非洲裔美国人）和960名 对照组（365名西班牙裔，340名白人和255名非洲裔美国人）。我们将 评估（1）与VDR的两种标志物（poly-A和FOKI）的相关性 西班牙裔和白人中的基因;（2）与BsmI/poly-A 单倍型在非洲裔美国人;和（3）可能的修改效果， 维生素D受体基因型与日光暴露和饮食的关系 维生素D摄入量我们还将储存DNA、血沉棕黄层和血浆， 未来的分子流行病学研究。建立在基础设施之上 在正在进行的研究中，我们有机会建立一个有价值的 更全面地评估维生素C和维生素D之间关系的资源 D和乳腺癌风险;用于进行未来的分子流行病学 未来将确定新的生物标志物和基因的研究; 以及研究多种族群体中基因与环境的相互作用 人口由于维生素D暴露是潜在的可改变的， 拟议的研究可能对新方法具有重要意义， 乳腺癌的预防

Experimental evidence and ecologic correlation studies support the hypothesis that vitamin D may reduce breast cancer risk. Yet this novel hypothesis is largely untested in human populations. An on-going population-based case-control study of breast cancer in Hispanic, African-American, and White Women will assess the associations with sunlight exposure and dietary vitamin D intake. On-going molecular research by one of the investigations of this applications suggests that polymorphisms of the vitamin D receptor (VDR) gene are related to the breast cancer risk. We therefore propose to expand the on-going case- control study in order to broaden our examination of the relation with vitamin D. In addition to the interview data being collected, we propose to obtain blood samples for a subset of study participants and to extend the case and control ascertainment by 10 months. Eligible cases will include women aged 35-79 and diagnosed with invasive breast cancer between May 1997 and February 1999. Cases will be identified through the cancer registries in the Greater San Francisco Bay area; controls will be selected from the general population through random-digit dialing. Professional interviewers will collect interview data, measure skin pigmentation, and obtain blood specimens through home visits. Blood specimens and risk factor data will be available for an estimated 640 cases (245 Hispanics, 225 Whites, and 170 African-Americans) and 960 controls (365 Hispanics, 340 Whites, and 255 African-Americans). We will assess (1) the association with two markers (poly-A and FOKI) of the VDR gene in Hispanics and Whites; (2) the association with the BsmI/poly-A haplotype in African-Americans; and (3) the possible modifying effect of VDR genotype on the association with sunlight exposure and dietary vitamin D intake. We will also bank DNA, buffy coat, and plasma for future molecular epidemiologic studies. Building upon the infrastructure of the on-going study, we have the opportunity to establish a valuable resource for more thoroughly assessing the relationship between vitamin D and breast cancer risk; for conducting future molecular epidemiologic research as new biomarkers and genes will be identified in the future; and for investigating gene-environment interactions in a multi-ethnic population. Since vitamin D exposure is potentially modifiable, the proposed research may have important implications for new approaches to breast cancer prevention.