MICROBIAL GENOMICS

对核梭杆菌和连翘拟杆菌基因组的完整DNA序列进行测定和分析，以确定基因产物的推定编码区和可能的功能。这两种细菌在口腔微生物学中很重要，包括牙周炎等疾病。它们已成为国家疾病预防控制中心口腔传染病战略规划讲习班进行DNA序列分析的目标。口腔传染病通常是多微生物性的，是由口腔微生物生态紊乱引起的。从正常口腔菌群的功能和它们之间的相互作用两方面了解这种生态，对于诊断、预防和治疗这些疾病至关重要。作为第一步，阐明口腔菌群重要成员的完整遗传内容将提供丰富的信息，从而对这些微生物的生理学、它们的毒力因素、生物膜形成机制以及它们与宿主和彼此相互作用的其他能力有更深入的了解。一旦基因组序列被确定和分析，它们将通过一个带有许多工具的在线数据库提供给研究界，以方便数据检索。在项目过程中，还将持续发布初步数据。

The complete DNA sequences of the genomes of Fusobacterium nucleatum and Bacteroides forsythus will be determined and analyzed for putative coding regions and possible functions of the gene products. These two bacteria are important in oral microbiology including diseases such as periodontitis. They have been targeted for DNA sequence analysis by a strategic planning workshop for oral infectious diseases of the NIDCR. Oral infectious diseases often are polymicrobial in nature and result from disturbances in the microbial ecology of the oral cavity. Understanding this ecology, both in terms of the capabilities of the normal oral flora as well as their interactions, is crucial to diagnosis, prevention, and cure of such diseases. As a first step, elucidating the complete genetic content of important members of the oral flora will provide a wealth of information that can lead to a profoundly deeper picture of the physiology of these microbes, their virulence factors, mechanism of biofilm formation, as well as their other capacities for interaction with the host and each other. Once the genomic sequences are determined and analyzed they will be made available to the research community through an online database with a number of tools to facilitate data retrieval. During the course of the project there will also be ongoing release of preliminary data.