DNA MICROARRAY INSTRUMENTATION

描述：(调查员？S摘要)：基于基因组的研究 过去的十年从根本上改变了我们进行生物医学研究的方法。 DNA微阵列是当前技术中最有潜力的 推进生物学研究。多个平台和协议正在 应用于微阵列技术的优化，以及不止一种这样的 事实证明，技术已经足够强大，可以在研究和 工业。许多VCU调查人员现在需要在他们的 研究项目。两个主要研究小组，一个由调查人员组成 一个是微生物应用，第二个是哺乳动物遗传学家和 生物化学家都参与其中。现有的商业布阵产品和服务 仍然极其昂贵、耗时，而且在许多情况下不适合 特定的应用程序。因此，VCU选择植入微阵列 在目前的核酸研究设施的保护伞下的设施。 这份提案代表了对两个核心的请求。所需仪器 要建立该设施，请执行以下操作： 一种BioRobotics公司的MicroGrid II阵列系统 GSI Lumonics ScanArray 4000标准生物芯片扫描系统 鉴于对该设施的迫切需求，VCU已获得 在副总统一级为以下方面提供重大机构支助 研究和健康科学系主任遍布全校。 通过这一机构支持，剩余的必要工具， 由液体搬运机器人(Qigen BioRobot 300(高装层))组成 高通量PCR反应的建立和处理)、高通量DNA 热循环仪(MJ Research Tetrad四头4 x 96/384反应仪)， 用于克隆存储的超低温冰柜和用于下游的软件 分析，将获得。机构承诺也随附于 以博士培训的科学家职位的形式提供的技术支持 在核酸研究设施中增加了一名技术员，并增加了一名 约500平方英尺的实验室，用于存放新仪器，并用于仪器的长期维护。

DESCRIPTION: (Investigator?s Abstract): Genome-based investigations over the past decade have fundamentally altered our approaches to biomedical research. DNA microarrays are foremost among current technologies in potential for advancing biological research. Multiple platforms and protocols are being applied in the optimization of microarray technology, and more than one such technology is proving sufficiently robust to have application in research and industry. Many VCU investigators now require microarray technologies in their research projects. Two main research groups, one consisting of investigators with microbial applications and a second comprised of mammalian geneticists and biochemists, are involved. Existing commercial arraying products and services remain extremely costly, time consuming and in many cases inappropriate for specific applications. Therefore, VCU has elected to implant a microarraying facility under the umbrella of the current Nucleic Acids Research Facility. This proposal represents a request for two of the ?core? instruments required to establish this facility: A BioRobotics, Inc. MicroGrid II Arraying System A GSI Lumonics ScanArray 4000 Standard Biochip Scanning System VCU, in view of the pressing requirements for this facility, has garnered significant institutional support at the level of the Vice President for Research and Health Sciences and the department chairs throughout the school. Through this institutional support the remaining required instrumentation, consisting of liquid handling robot (Qiagen BioRobot 300 (decked out for high throughput PCR reaction set up and processing), high throughput DNA thermocycler (MJ Research Tetrad four head 4 x 96/384 reaction instrument), ultralow temperature freezer for clone storage, and software for downstream analysis, will be acquired. Institutional commitments are also attached for technical support in the form of a position for a Ph.D. trained scientist, an additional technician in the Nucleic Acids Research Facility, and an additional approximately 500 ft2 laboratory to house the new instruments, and for the long term maintenance of the instruments.