CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE IN NEUROTRANSMITTER RELEASE MODULATION

Ca 2 +/钙调蛋白依赖性蛋白激酶II磷酸化 突触蛋白I磷酸化的突触蛋白I从突触膜上解离， 囊泡并引起神经递质的释放。 两种形式的 Ca 2 +/钙调蛋白依赖性蛋白激酶II已被鉴定为 可溶形式和突触囊泡相关形式。 的 囊泡相关形式的激酶既作为结合蛋白， 突触蛋白I的蛋白质，并促进其从 囊泡 这两种形式的激酶之间的关系是 不清楚 在本研究中，我们试图使用质量 光谱技术来表征这两种形式的结构 通过测量它们的质量和获得它们的肽图来研究激酶。

Ca2+/calmodulin-dependence protein kinase II phosphorylates synapsin I. The phosphorylated synapsin I dissociates from synaptic vesicle and causes the release of neurotransmitter. Two forms of Ca2+/calmodulin-dependent protein kinase II have been identified the soluble form and the synaptic vesicle-associated form. The vesicle-associated form of the kinase functions both as a binding protein for synapsin I and promotes its dissociation from the vesicles. The relationship between these two forms of the kinases is not clear. In the present study, we are attempting to use mass spectrometric techniques to structurally characterize these two forms of kinases by measuring their masses and obtaining their peptide maps.