MEMBRANE PROTEIN STRUCTURE : USE OF LIPIDIC MESOPHASES

描述：(改编自申请)本修订申请旨在 将卡弗里博士在脂类方面的专业知识应用于立方脂类的合理设计 膜蛋白结晶的阶段。虽然有一些人 膜蛋白，特别是细菌视紫红质(BR)已被成功地 从脂质立方中间相结晶而来，还没有仔细的研究 中间相的最佳状态，以及中间相之间边界的细节 生长中的晶体和块体相。相反，只有经验性的程序是 用来获得晶体的。为了补救这一点，卡弗里博士提议延长他的 形成立方中间相的单酰甘油相图图库， 并研究了相图与最佳相图的关系。 BR和细菌光反应中心RC的结晶。结果是 这些研究，以及对晶体/脂质的详细分析 接口然后将用于尝试合理地结晶 衣藻先前未结晶的光系统II。

Description: (Adapted from the application) This revised application aims to apply Dr. Caffrey's expertise in lipids to the rational design of cubic lipid phases for the crystallization of membrane proteins. Though a number of membrane proteins, notably bacteriorhodopsin (BR), have been successfully crystallized from lipid cubic mesophases, there has been no careful study of the optimum state of the mesophase, and of the details of the boundary between the growing crystal and the bulk phase. Instead only empirical procedures are used to obtain crystals. To remedy this, Dr. Caffrey proposes to extend his library of phase diagrams for monoacylglycerols, which form cubic mesophases, and to study the relationship between the phase diagrams and the optimum crystallization of BR and of a bacterial photoreaction center, RC. The results of these studies, together with an analysis in detail of the crystal/lipid interface will then be used in attempts at rational crystallization of the previously uncrystallized photosystem II from Chlamydomonas.