MOLECULAR ASPECTS OF HUMAN LYMPHOPOIESIS

这个新的计划项目将联合四个既定的研究人员在人类免疫系统的发展和衰老的研究。该计划项目是从Kincade博士，Thompson博士和Webb博士的认识发展而来的，他们认识到人类缺乏小鼠免疫系统的许多非常基本的信息，这使得他们很难将他们最近在小鼠身上取得的令人兴奋的发现扩展到人类。然而，新技术使他们很难将他们最近在小鼠身上取得的令人兴奋的发现扩展到人类身上。然而，新的技术和模型系统现在使得利用人类淋巴细胞前体和成熟细胞的次要亚群进行分子研究变得可行。第四位研究者Capra博士已经并将继续专注于人类体液免疫反应。这四个项目是高度集成的，涵盖了淋巴细胞发育的许多方面。汤普森和金凯德博士将分别研究T和B淋巴细胞分化的早期事件。Kincade博士和Capra博士将研究衰老对B细胞发育的影响，Kincade博士的研究重点是早期阶段，Capra博士的研究重点是改变抗体形成阶段。WebB博士将研究B细胞转录因子在形成期所有阶段的表达和功能。WebB博士将研究B细胞转录因子在形成期所有阶段的表达和功能。Dr. WebB将研究B细胞转录因子在B细胞分化的所有阶段的表达和功能，目的是了解其在X连锁a γ球蛋白血症中的作用。所有四个项目都涉及人类淋巴细胞离散群体的表征和/或使用，使带有新型MoFlo流式细胞仪的流式细胞仪核心成为该计划项目的组成部分。四位研究人员将分享稀有细胞群以及用于表征它们的分子技术。该方案项目的顺利运作也通过一个行政核心得到保证。四个项目负责人的共同努力将产生新的见解，在人类淋巴细胞发育和异常的免疫缺陷，自身免疫，恶性肿瘤和衰老中发生的分子事件。

This new Program Project will unite four established investigators in studies of the development and senescence of the human immune system. The Program Project evolved from the realization by Drs. Kincade, Thompson, and Webb that much very basic information available for the murine immune system is lacking for humans, making it difficult for them to extend their recent exciting findings made with mice to humans. However, new technologies make it difficult for them to extend their recent exciting findings made with mice to humans. However, new technologies and model systems now make it feasible to undertake molecular studies with human lymphocyte precursors and minor subsets of mature cells. Dr. Capra , the fourth investigator, was already, and will continue to be, focused on the human humoral immune response. The four projects are highly integrated and cover many aspects of lymphocyte development. Drs. Thompson and Kincade will study early events in the differentiation of T and B lymphocytes, respectively. Drs. Kincade and Capra will examine the effects of aging on B cell development with Dr. Kincade's efforts focused on early stages and Dr. Capra's on the alter antibody-forming phases. Dr. Webb will study the expression and function of a B cell transcription factor at all stages of forming phases. Dr. Webb will study the expression and function of a B cell transcription factor at all stages of forming phases. Dr. Webb will study the expression and function of a B cell transcription factor at all stages of B cell differentiation with a goal of understanding its role in X-linked a gamma globulinemia.. All four projects involve characterization and/or use of discrete populations of human lymphocytes, making the Flow Cytometry Core with a new MoFlo flow cytometer an integral part of the Program Project. The four investigators will share rare populations of cells as well as molecular techniques for their characterization. The smooth functioning of the Program Project is also assured through an Administrative Core. The combined efforts of the four Project Leaders will yield new insights into the molecular events in human lymphocyte development and abnormalities which occur in immunodeficiencies, autoimmunity, malignancies, and aging.