SPECTROSCOPY FOR ALZHEIMER'S DISEASE DIAGNOSIS

描述(逐字摘自申请人的摘要)：建议的研究 这里将导致发展出一种实用、无创、比较 利用近红外诊断阿尔茨海默病(AD)的廉价方法 (近红外)低能量激光光谱学。AD是光谱学的理想候选者 诊断。已证明存在独特的生化标志物(老年人 富含淀粉样蛋白纤维和神经纤维缠结的斑块 磷酸化蛋白质)，将提供独特的光谱特征。 研究人员开发脑氧监测器并研究大脑光学 断层扫描显示，脑组织对近红外是高度半透明的。 波长，并且足够的光可以通过 用于诊断目的的完整的成人头骨。海拔最高的地区 AD标志物集中在颞叶，位于太阳穴下方 头骨最薄的地方。使用的激光能量是安全的，组件 产生和检测这些波长所需的资源是现成的。这个 该装置旨在用于床边，以测量程度 疾病，一种决定建议的疗效的基本能力 治疗和监测疾病进展情况。这项提议代表着 麻省理工学院激光生物医学研究中心(LBRC)与领先的 在光谱诊断研究和贝德福德退伍军人医学中心(VAMC)， AD研究的领先者。LBRC成功证明了癌症的诊断 在不同的器官使用激光诱导的荧光(口腔，结肠，膀胱， 乳房)和冠状动脉定量生化分类 堵塞和血液分析(葡萄糖、白蛋白)，使用拉曼散射。 在LBRC进行的比较正常和AD标本的初步研究 由VAMC提供的颞叶皮质和颞骨(包括显微镜和 和大块样品)，在荧光和 拉曼光谱。这项工作将建立适当的签名选择， 设计临床系统所需的波长和算法。特别是，一个 近红外荧光光谱分析的体外前瞻性研究 检测人类脑组织中的阿尔茨海默病。这项工作将建立适当的 设计临床所需的签名、波长和算法的选择 系统。

DESCRIPTION (Verbatim from the Applicant's Abstract): The research proposed here will lead to the development of a practical, non-invasive, comparatively inexpensive method for diagnosing Alzheimer's disease (AD) using near-infrared (NIR) low energy laser spectroscopy. AD is an ideal candidate for spectroscopic diagnosis. The demonstrated presence of unique biochemical markers (senile plaques containing amyloid fibrils and neurofibrillary tangles rich in phosphorylated proteins), will provide unique spectroscopic signatures. Researchers developing cerebral oxygen monitors and studying brain optical tomography have shown that brain tissue is highly translucent to NIR wavelengths and that sufficient light can be transmitted and detected through an intact adult skull for diagnostic purposes. The region of highest concentration of AD markers is the temporal lobe, located beneath the temple where the skull is thinnest. The laser energy used is safe, and the components needed to generate and detect these wavelengths are readily available. The device is intended for use at the bedside in order to measure the degree of disease, an essential capability for determining the efficacy of proposed curative treatments and monitoring disease progress. This proposal represents a collaboration between the MIT Laser Biomedical Research Center (LBRC), a leader in spectroscopic diagnostic research and the Bedford VA Medical Center (VAMC), a leader in AD research. LBRC has successfully demonstrated diagnosis of cancer in various organs using laser induced fluorescence (mouth, colon, bladder, breast), and quantitative biochemical classification of coronary artery blockages and blood analytes (glucose, albumin), using Raman scattering. Preliminary studies performed at the LBRC comparing specimens of normal and AD temporal cortex and temporal bone, provided by VAMC (including both microscopic and bulk samples), show very promising signatures in both fluorescence and Raman spectra. This work will establish the appropriate choices of signatures, wavelengths and algorithms needed to design a clinical system. In particular, a prospective study in vitro demonstrated near-infrared fluorescence spectroscopy detects AD in human brain tissue. This work will establish the appropriate choices of signatures, wavelengths and algorithms needed to design a clinical system.