CORNEAL EXTRACELLULAR MATRIX METALLOPROTEINASES

过度的细胞外基质金属蛋白酶（MMPs）活性 会导致角膜溃疡。 瘢痕形成，由于 角膜溃疡是失明和视力受损的主要原因。 我们关注常用眼科药物的效果 和紫外线照射对角膜基质金属蛋白酶的影响。 我们建议研究四大 角膜基质金属蛋白酶：明胶酶A、明胶酶B、胶原酶和基质溶解素 在器官培养中的正常、损伤和患病条件下。 猪 将使用角膜。 眼部药物和紫外线辐射的影响 将评估这些MMP中的每一种的净合成。 的 待测试的药物包括抗菌剂， 抗真菌药，抗组胺药，瞳孔扩张剂，抗青光眼药， 抗胆碱能和抗炎。 这些影响 每种纯化的MMPs的活性的药物也将被 研究了 我们工作的结果可能会导致更好的理解 这些药物和紫外线辐射的结构完整性 角膜 我们的长期目标是确定 环境因素对角膜结构完整性的影响。

Excessive extracellular matrix metalloproteinases (MMPs) activities in the cornea can lead to cornea ulceration. Cicatrization due to cornea ulceration is a major cause of blindness and impaired vision. We are concerned about the effect of commonly used eye medications and UV radiation on corneal MMPs. We propose to study the four major corneal MMPs: gelatinase A, gelatinase B, collagenase and stromelysin under normal, injured and diseased conditions in organ culture. Pig corneas will be used. The effect of eye medications and UV radiation on the net synthesis of each of these MMPs will be evaluated. The medications to be tested include agents for antibacterial, antifungus, antihistamine, pupil dilator, antiglucoma, anticholinergic and anti-inflammatory. The effect of these medications of the activity of each purified MMPs will also be studied. The results of our work may lead to a better understanding of these medications and UV radiation on the structural integrity of the cornea. Our long term goal is to determine the effect of environmental agents on the structural integrity of the cornea.