Bacillus anthracis clone set

项目描述（申请人提供）：本项目的长期目标是克隆并在大肠杆菌中表达炭疽杆菌（炭疽的病原体）的所有基因。这种病原体具有严重的生物战威胁，但由于自然感染的发生率较低，研究相对不足。重要的第一步是确定所有抗原蛋白、候选疫苗、治疗靶点，并根据DNA序列差异和免疫反应开发诊断方法。所有这些目标都可以从在大肠杆菌等安全、易于操作的替代宿主中适当表达的所有基因的克隆集开始实现。在本项目一期，包括本申请，将克隆和表达两个毒力质粒的基因和有限数量的染色体基因，并用免疫学试剂进行探测。

DESCRIPTION (provided by applicant): The long-term aim of this project is to clone and express in E. coli all the genes from Bacillus anthracis, the causative agent of anthrax. This pathogen poses a serious biowarfare threat yet has been relatively understudied due to the low incidence of natural infection. An important first step would be to identify all antigenic proteins, vaccine candidates, therapeutic targets, and develop diagnostics based on DNA sequence differences and immunological responses. All of these goals can be met starting with a clone set of all genes appropriately expressed in a safe, easy to manipulate surrogate host like E. coli. During Phase I of this project, covered by the present application, genes from the two virulence plasmids and a limited number of chromosomal genes will be cloned and expressed and probed with immunological reagents.