SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON

描述：发现了两种新的酵母蛋白质，Duo 282 p， 基本的纺锤体成分，以及Jsn 1 p，一种似乎影响 微管动力学并在微管亲和柱上富集， 提供了独特的机会，以确定如何辅助蛋白调节 微管在体内组装的动力学。 此外，第三种蛋白质 这似乎提供了一个功能之间的联系， 微管和肌动蛋白细胞骨架。 这种蛋白质Cor 1 p 显然是微管和皮层肌动蛋白之间的联系 细胞骨架在纺锤体方向。 本提案的重点是 研究这些新蛋白质影响 微管动力学和纺锤体功能。 具体目标是：（1） 阐明Cor 1 p与肌动蛋白相互作用的性质和功能， 微管细胞骨架，并从遗传学和生物化学上鉴定 与酵母Cor 1 p相互作用的蛋白质;（2）确定特定的 酵母Duo 282 p在纺锤体功能和组装中的功能，并确定 （3）阐明Jsn 1 p与纺锤体相互作用的性质; 通过鉴定具有以下功能的蛋白质来调节微管动力学： 与Jsn 1 p并行。 总的来说，这些研究将定义组件 微管和肌动蛋白细胞骨架， 染色体遗传

DESCRIPTION: The discovery of two novel yeast proteins, Duo282p, an essential spindle component, and Jsn1p, a protein that appears to influence microtubule dynamics and is enriched on microtubule affinity columns, provides unique opportunities to determine how accessory proteins regulate the dynamics of microtubule assembly in vivo. In addition, a third protein has been discovered which appears to provide a functional link between the microtubule and actin cytoskeletons during mitosis. This protein, Cor1p apparently serves as a link between microtubules and the cortical actin cytoskeleton during spindle orientation. The focus of this proposal is to investigate the mechanisms by which these novel proteins influence microtubule dynamics and spindle function. The specific aims are to: (1) elucidate the nature and function of Cor1p's interaction with the actin and microtubule cytoskeletons and to genetically and biochemically identify proteins which interact with yeast Cor1p; (2) determine the specific function of yeast Duo282p in spindle function and assembly and determine the nature of its interaction with spindles; (3) elucidate the role of Jsn1p in regulating microtuble dynamics by identifying proteins which function with or in parallel with Jsn1p. In total, these studies will define components of the microtubule and actin cytoskeletons that are required to ensure proper chromosome inheritance.