Dynamic Vascular Functional Imaging: Optical Tomography

描述(逐字摘自申请者摘要)：在本建议书中，我们介绍 动态血管功能成像(DVFI)作为一种新的能力 一种研究人体血红蛋白状态时空特性的方法 大的组织结构。我们开发的方法使用非电离 近红外光源可进行快速、多波长、层析成像 测量，由此产生横截面图像的时间序列。 我们假设对这些数据的分析，使用适当的线性和非线性 时间序列分析方法，能够揭示重要的函数关系 血管树的特征，特别是与血管树相关的特征 微血管系统。功能强大、通用、快速的光学成像硬件 我们的设计能够检查几乎所有的解剖部位 光学穿透的限制(15厘米厚度)已经到位。也是 提供一系列映像恢复和分析软件，并且经过了良好的测试 非常适合于动态成像数据的研究。我们展示了广泛的 初步证据证明了我们的探测和区分能力 与血红蛋白状态测量相关的复杂时空动力学 (例如，血容量和血红蛋白氧饱和度水平) 前臂和其他介质的全横断面图。 拟议研究的目标是进行可行性研究 确定我们的成像技术是否适合于 调查前臂肌肉可能存在的功能缺陷 其他健康的受试者和那些患有2型糖尿病的人 50岁。对于每个受试者组，我们将计算不同的时空 从基线研究收集的成像数据和从 为回应特定挑衅而收集的数据。这些数据将是 评估它们与已知解剖标志和特定 与血管反应性相关的功能特性。补充这些内容 调查将是涉及前臂磁共振图的数值研究，以及 在实验室模体上进行的测量，旨在识别 可以检测不同时空特征的精度以及 差异化。

DESCRIPTION (Verbatim from Applicant's Abstract): In this proposal we introduce the capability of dynamic vascular functional imaging (DVFI) as a novel approach to investigate the spatio-temporal properties of hemoglobin states in large tissue structures. The methodology we have developed employs non-ionizing near infrared optical sources to perform fast, multi-wavelength, tomographic measurements, from which a time series of cross-sectional images are generated. We hypothesize that analysis of these data, using suitable linear and nonlinear time-series analysis methods, is capable of revealing important functional features of the vascular tree, in particular that associated with the microvasculature. Functioning, general purpose, fast optical imaging hardware of our design that is capable of examining nearly any anatomical site within the limits of optical penetration (15 cm thickness) is already in place. Also available, and well tested, is an array of image recovery and analysis software well suited for the investigation of dynamic imaging data. We present extensive preliminaiy evidence documenting our ability to detect and differentiate complex spatio-temporal dynamics associated with measures of hemoglobin states (e.g., blood volume and hemoglobin oxygen saturation levels) in the human forearm and other media in a full cross-sectional view. The objective of the proposed research is to conduct a feasibility study for the purpose of identifying the suitability of our imaging technology to investigate possible functional deficits in muscle of the forearm from otherwise healthy subjects and from those with type-2 diabetes who are at least 50 years of age. For each subject group we will compute various spatio-temporal dynamic features from the imaging data collected from baseline studies and from data collected in response to specific provocations. These data will be evaluated for their correlations with known anatomical landmarks and specific functional properties associated with vascular reactivity. Supplementing these investigations will be numerical studies involving MR maps of the forearm, and measurements performed on laboratory phantoms intended to identiying limits of the accuracy by which different spatio-temporal features can be detected and differentiated.