XRAY DATA COLLECTION OF RNA POLYMERASE II & DNA CO CRYSTAL

RNA聚合酶II/DNA共晶体是最近用 旋转阳极X射线源对5E的衍射。鉴于 旋转阳极X射线的5E分辨率显著提高 在一项关于聚合酶的平行研究中，同步加速器的3E 仅此一项，共晶就有望通过同步加速器衍射到3E 波姆也是。一种冷冻程序已经被开发出来， 使从一块晶体中收集数据成为可能。我们现在建议 收集聚合酶II/DNA的高分辨率X射线衍射数据 钴晶体及其重原子衍生品。我们的目标是 获得用于确定晶体的高质量的衍射数据 RNA聚合酶II/DNA复合体的结构。

Cocrystal of RNA polymerase II/DNA has been recently obtained with diffraction to 5 E on rotating-anode X-ray source. In view of the significant improvement from 5 E resolution with rotating-anode X-ray to 3 E at the synchrotron source in a parallel study of the polymerase alone, the cocrystal is expected to diffract to 3 E with synchrotron beam as well. A cryosoaking procedure has already been developed, making data collection from one crystal feasible. We now propose to collect high resolution X-ray diffraction data on polymerase II/DNA cocrystal as well as its heavy atom derivatives. The goal is to obtain high quality diffraction data for determining the crystal structure of RNA polymerase II/DNA complex.