PROTEIN/PROTEIN INTERACTIONS OF HIV-1 INTEGRASE

描述（改编自研究者摘要）：本项目提出 研究HIV整合酶与宿主的蛋白质相互作用 蛋白 在以前的研究中，PI使用酵母两个 用杂交系统分离HIV整合酶缺陷突变体 低聚反应她还使用了两个混合系统来识别 一种与HIV-1整合酶紧密结合的蛋白质Ini-1。Ini-1圈 在序列上与Snf-5相似，Snf-5是Swi/Snf的一个组成部分， 复合物被认为与基因激活有关。PI计划 研究异聚体蛋白质间的相互作用 通过酵母双杂交系统的进一步实验。突变株 影响这些相互作用及其表型 在体外和体内研究。Ini-1对靶位点的影响 还将研究逆转录病毒整合过程中的选择， Ini-1在HIV生命周期中的作用希望结果 这些研究将有助于设计整合抑制剂， 开发新的基因治疗方法。

DESCRIPTION (Adapted from investigator's abstract): This project proposes to investigate protein-protein interactions of HIV integrase with host protein. In previous studies, the PI used the yeast two hybrid system to isolate mutants of HIV integrase defective in oligomerization. She has also used the two hybrid system to identify a protein, Ini-1, that binds tightly to HIV-1 integrase. Ini-1 turns out to be similar in sequence to Snf-5, a component of the Swi/Snf complex proposed to be involved in gene activation. The PI plans to investigate heteromeric protein-protein interactions by further experiments using the yeast two hybrid system. Mutants will be generated that influence these interactions, and their phenotypes studied in vitro and in vivo. The influences of Ini-1 on target site selection during retroviral integration will also be investigated, as will the role of Ini-1 in the HIV life cycle. It is hoped that results of these studies will be useful in devising integration inhibitors and in developing new gene therapy methods.