Vitrified Immobilized Proteins and Ligands

描述（由申请人提供）：本研究的总体目标是 开发一种新的工艺和实验室仪器， 玻璃化固定蛋白质和配体的微点。固定化蛋白质 而储存在玻璃化的糖基质中的配体将被很好地保存下来 在室温甚至高温下。产生这种微斑点的能力 可以在性能、成本、便利性和 需要固定化蛋白质的应用的储存要求， 配体。可以改进的应用实例包括免疫测定， 使用固定化酶的合成、涉及亲和性的纯化 色谱法和使用固定化抗体、酶和配体的传感器。 我们建议使用微喷射打印机，指定SCAMP，创建玻璃化 微点我们开发了SCAMP作为实验室仪器来打印肽 微阵列我们建议修改SCAMP以打印蛋白质/糖溶液， 确定产生玻璃化微点的条件。微点 最初打印的尺寸对于喷墨打印机是典型的：100 皮升体积，产生直径为60 - 100微米的斑点。的 还将确定使用该技术的优点和缺点。 拟议的商业应用： 所提出的方法可以导致改进的免疫测定，合成使用固定化酶，纯化涉及亲和色谱，和传感器使用固定化抗体，酶和配体。

DESCRIPTION (provided by applicant): The overall objective of this research is to develop a new process and laboratory instrument capable of synthesizing microspots of vitrified immobilized proteins and ligands. Immobilized proteins and ligands stored in a vitrified (glassy) sugar matrix would be well preserved at room and even elevated temperatures. The ability to create such microspots could generate significant improvements in performance, cost, convenience, and storage requirements for applications requiring immobilized proteins and ligands. Examples of applications that could be improved include immunoassays, syntheses using immobilized enzymes, purifications involving affinity chromatography, and sensors using immobilized antibodies, enzymes, and ligands. We propose to use a microjet printer, designated SCAMP, to create vitrified microspots. We developed SCAMP as a laboratory instrument to print peptide microarrays. We propose to modify SCAMP to print protein/sugar solutions, and determine the conditions that yield vitrified microspots. The microspots printed would initially be of dimensions typical for an ink jet printer: 100 picoliters volume, creating a spot 60 - 100 micrometers in diameter. The advantages and drawbacks in using this technology will also be determined. PROPOSED COMMERCIAL APPLICATIONS: The proposed method could lead to improved immunoassays, syntheses using immobilized enzymes, purifications involving affinity chromatography, and sensors using immobilized antibodies, enzymes, and ligands.