Dynamic Vascular Functional Imaging: Optical Tomography

描述（来自申请人摘要的逐字）：在本提案中，我们介绍 动态血管功能成像（DVFI）作为一种新的 一种研究血红蛋白状态的时空特性的方法， 大型组织结构。我们开发的方法采用非电离 近红外光源进行快速，多波长，断层扫描 测量，从该测量生成横截面图像的时间序列。 我们假设，这些数据的分析，使用适当的线性和非线性 时间序列分析方法，能够揭示重要的功能 血管树的特征，特别是与血管相关的特征， 微脉管系统功能强大的通用快速光学成像硬件 我们的设计，能够检查几乎任何解剖部位， 光学穿透的极限（15厘米厚）已经存在。也 一系列经过良好测试的图像恢复和分析软件 非常适合于动态成像数据的研究。我们提供广泛的 有充分的证据证明我们有能力发现和区分 与血红蛋白状态测量相关的复杂时空动态 (e.g.,血容量和血红蛋白氧饱和度水平 前臂和其他媒体在一个完整的横截面视图。 拟议研究的目的是进行可行性研究， 确定我们的成像技术的适用性的目的， 研究前臂肌肉可能的功能缺陷， 其他健康受试者和2型糖尿病患者， 五十岁。对于每个主题组，我们将计算各种时空 从基线研究和基线研究收集的成像数据的动态特征 针对具体挑衅行为收集的数据。这些数据将 评价其与已知解剖标志和特定 与血管反应性相关的功能特性。补充这些 调查将是涉及前臂MR图的数值研究， 在实验室体模上进行的测量，旨在确定 可以检测不同时空特征的精度，以及 差异化。

DESCRIPTION (Verbatim from Applicant's Abstract): In this proposal we introduce the capability of dynamic vascular functional imaging (DVFI) as a novel approach to investigate the spatio-temporal properties of hemoglobin states in large tissue structures. The methodology we have developed employs non-ionizing near infrared optical sources to perform fast, multi-wavelength, tomographic measurements, from which a time series of cross-sectional images are generated. We hypothesize that analysis of these data, using suitable linear and nonlinear time-series analysis methods, is capable of revealing important functional features of the vascular tree, in particular that associated with the microvasculature. Functioning, general purpose, fast optical imaging hardware of our design that is capable of examining nearly any anatomical site within the limits of optical penetration (15 cm thickness) is already in place. Also available, and well tested, is an array of image recovery and analysis software well suited for the investigation of dynamic imaging data. We present extensive preliminaiy evidence documenting our ability to detect and differentiate complex spatio-temporal dynamics associated with measures of hemoglobin states (e.g., blood volume and hemoglobin oxygen saturation levels) in the human forearm and other media in a full cross-sectional view. The objective of the proposed research is to conduct a feasibility study for the purpose of identifying the suitability of our imaging technology to investigate possible functional deficits in muscle of the forearm from otherwise healthy subjects and from those with type-2 diabetes who are at least 50 years of age. For each subject group we will compute various spatio-temporal dynamic features from the imaging data collected from baseline studies and from data collected in response to specific provocations. These data will be evaluated for their correlations with known anatomical landmarks and specific functional properties associated with vascular reactivity. Supplementing these investigations will be numerical studies involving MR maps of the forearm, and measurements performed on laboratory phantoms intended to identiying limits of the accuracy by which different spatio-temporal features can be detected and differentiated.