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傅里叶变换红外光谱(FT-IR)正在发展中 作为糖基组成分析的独立方法和作为 毛细管电泳法的辅助分离 糖共轭化合物的单糖组成。初步结果表明 傅立叶变换红外光谱可用于区分不同种类的 单糖异构体的光谱，以及通常使用的缓冲液 对于CE，分离不会干扰红外检测。最近， 发展了一种新的红外光谱定量实验方法 用FT-IR显微镜进行分析。样品的薄膜光谱 在锌硒平板上沉积和干燥的溶液用 FT-IR显微镜和高灵敏度碲化镉汞 探测器。一个相当广泛的单糖红外光谱库 在不同条件下以高分辨率采集。这个 文库正在扩展，以包括双低聚糖和更高级的寡糖。 最近的工作集中在执行糖基化的潜在功能上。 红外光谱分析Per-N，O-乙酰化产物的组成 得到单糖甲基糖苷混合物的衍生物 在单糖和低聚糖的酸性甲烷化后。这个 在甲烷分解后引入过乙酰化步骤是因为 观察到单糖甲基糖苷沉积较差。 特点。由于该方法的成功应用将 依赖于来自广泛的训练频谱的先前获取 已知样品的分布，包括纯单糖 作为标准混合物，产生了大量这样的样品 由已知的N-和O-连接的糖的组合 哺乳动物糖蛋白的寡糖。来自145的光谱 已获得已知组成的标准，该方法是 现在正在对未知的寡糖和单糖进行测试 混合物。

Fourier transform-infrared (FT-IR) spectroscopy is being developed as a stand-alone method for glycosyl composition analysis and as an adjunct to capillary electrophoretic (CE) separation of the monosaccharide components of glycoconjugates. Early results indicated that FT-IR spectroscopy can be used to differentiate among a wide spectrum of monosaccharide isomers, and that buffers typically used for CE separations do not interfere with IR detection. More recently, a new experimental method has been developed for FT-IR quantitative analysis with the use of FT-IR microscopy. Film spectra of sample solutions deposited and dried on a ZnSe plate were collected with an FT-IR microscope and a high-sensitivity mercury cadmium telluride detector. A fairly extensive library of monosaccharide IR spectra has been collected at high resolution under different conditions. The library is being extended to include di- and higher oligosaccharides. Recent work has focused on the pote ntial of performing glycosyl composition analysis by FT-IR spectroscopy of per-N,O-acetylated derivatives of the monosaccharide methylglycoside mixtures obtained after acidic methanolysis of mono- and oligosaccharides. The peracetylation step was introduced after methanolysis because the monosaccharide methylglycosides were observed to have poor deposition characteristics. Since succesful application of the method will depend on prior acquisition of training spectra from a wide distribution of known samples, including pure monosaccharides as well as standard mixtures, a large number of these samples were generated from combinations of sugars known to comprise N- and O-linked oligosaccharides of mammalian glycoproteins. Spectra from 145 standards of known composition have been acquired, and the method is now being tested on unknown oligosaccharides and monosaccharide mixtures.