CORE--LABORATORY

为了支持儿童健康中心，我们建议建立 由Bertram Lubin博士领导的核心实验室和 连接到儿童医院奥克兰研究所(CHORI)。 核心实验室的具体目标是：(1)保持 将执行必要的实验室功能的集中式设施 研究中心的研究部分，包括建立 实验室收集、贴标签、搬运和储存规程 所有生物和环境标本；(2)妥善管理 从Salinas现场送来的生物和环境样本以及 将它们储存起来以备将来使用，并送到参考实验室 (3)按照中心要求进行农药分析(概述如下)； 监督测试现场的样品采集，并确保适当的 处理和运送样本至核心设施；及(4) 输入有关生物和环境样本的信息 一个中央数据系统，用于跟踪、回收样品，以及 信息的传递。 为了实现具体目标1，我们将安装必要的设备并购买 完成所需测试所需的孩子，不包括分析 尿液和环境样本中的杀虫剂。达到特定目标 2、Core的一名实验室技术人员将接收、存储和发货 样本并确认从每个人身上收集的所有样本 这项研究。对于具体目标3，核心实验室中给出的具体内容 应用说明收集和运输所使用的方法 卢宾博士将监督这些细节的实施。至 为实现具体目标4，我们将建立一个计算机数据库系统，以 由核心实验室技术人员使用，并能够跟踪收集 所有样本和每个样本在整个过程中的处理 这项研究。 建立实验室分析核心是有效利用 资源AS CHORI已经能够执行许多分析 是必需的，并且可以这样做而不会招致外部的费用 实验室。这项研究的规模和多个地点 堆芯中的生物和环境样本将创造一个 进一步研究农场工人人口的重要资源 接触杀虫剂的儿童人口。

In order to support the Children's Health Center, we propose to establish a Core laboratory under the directorship of Dr. Bertram Lubin and connected to the Children's Hospital Oakland Research Institute (CHORI). The specific aims of the Core laboratory are: (1) to maintain a centralized facility that will perform necessary laboratory functions for research for research components of the center, including establishing laboratory protocols for the collection, labelling, handling and storage of all biologic and environmental specimens; (2) to appropriately manage biological and environmental samples sent from the Salinas site and either store them for future use, send them to reference laboratories for pesticide analysis as required for the center (outlined below); (3) to monitor collection of samples at test sites and to ensure appropriate handling and transportation of samples to the core facility; and (4) to enter information concerning both biological and environmental samples in a central data system to enable tracking, recovery of samples, and transmission of information. To meet specific aim 1, we will set up the necessary equipment and buy the necessary kids for performance of the required tests, excluding analysis of urine and environmental specimens for pesticides. To meet specific aim 2, a laboratory technician at the Core will receive, store, and ship each specimen and confirm the collection of all samples from each individual in the study. For specific aim 3, the specifics given in the Core laboratory application describe methods to be used for collection and transportation of specimens; Dr. Lubin will oversee implementation of these specifics. To meet specific aim 4, we will establish a computer data base system to be used by the Core laboratory technician and capable of tracking collection of all specimens and handling of each specimen throughout the course of the study. Establishing a Core for laboratory analysis is an efficient use of resources as CHORI is already capable of performing many of the assays required and can do so without incurring the expenses of an outside laboratory. The magnitude of this study and the multiple sites of biological and environmental specimens in the Core will create an important resource for further study of a farmworker population and a population of children exposed to pesticides.