FREE SOLUTION MOBILITY AS A PROBE OF DNA ELECTROSTATICS

描述：这一修改后的新申请寻求资金来刻画 用自由溶液迁移率观察DNA的静电学特征 一些DNA分子。第一个目标是衡量网络的效果 电场中DNA迁移的电荷。第二个是 研究DNA上有效电荷的序列依赖性 分子，后续的初步工作表明，情况就是这样。 结合FRET分析，提出的实验将挑战 DNA小沟中单价反离子结合的假说 静电崩塌并导致向小凹槽弯曲。进一步 这项工作需要研究DNA和常用药物之间的相互作用 电泳法实验中的缓冲器。

DESCRIPTION: This amended new application seeks funding to characterize features of DNA electrostatics by observation of the free solution mobilities of a number of DNA molecules. The first goal is to measure the effect of net charge on the migration of DNA in an electric field. The second is to investigate the sequence dependence of the effective charge on the DNA molecule, following up on preliminary work indicating that this is the case. Combined with FRET analysis, the experiments proposed will challenge the hypothesis that monovalent counterion binding in the minor groove of DNA causes electrostatic collapse and leads to bending towards the minor groove. Further work entails an examination of the interaction between DNA and commonly used buffers in electrophoresis experiments.