FREE SOLUTION MOBILITY AS A PROBE OF DNA ELECTROSTATICS

作为 DNA 静电探针的自由溶液迁移率

• 批准号: 6693428
• 负责人: NANCY C STELLWAGEN
• 金额: $ 25.03万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6693428
• 关键词: DNA; buffers; capillary electrophoresis; chemical property; circular dichroism; conformation; electric field; electrical conductance; electrical impedance; electrical measurement; electrical property; electrochemistry; fluorescence resonance energy transfer; fluorescent dye /probe; ionic bond; ionic strengths; molecular asymmetry; molecular shape; nucleic acid structure; oligonucleotides; polyion

DESCRIPTION: This amended new application seeks funding to characterize features of DNA electrostatics by observation of the free solution mobilities of a number of DNA molecules. The first goal is to measure the effect of net charge on the migration of DNA in an electric field. The second is to investigate the sequence dependence of the effective charge on the DNA molecule, following up on preliminary work indicating that this is the case. Combined with FRET analysis, the experiments proposed will challenge the hypothesis that monovalent counterion binding in the minor groove of DNA causes electrostatic collapse and leads to bending towards the minor groove. Further work entails an examination of the interaction between DNA and commonly used buffers in electrophoresis experiments.

描述：此修订后的新申请寻求资金，以表征 DNA静电特性的自由溶液迁移率观察 一系列DNA分子。第一个目标是衡量网络的效果 电荷对DNA在电场中迁移的影响。二是 研究DNA上有效电荷的序列依赖性 分子，后续的初步工作表明，这是情况。 结合FRET分析，提出的实验将挑战 在DNA小沟中结合单价互补物导致 静电崩溃并导致朝向小沟弯曲。进一步 这项工作需要检查DNA和常用的DNA之间的相互作用。 电泳实验中的缓冲液。

项目成果

Effect of the matrix on DNA electrophoretic mobility.

• DOI: 10.1016/j.chroma.2008.11.090
• 发表时间: 2009-03-06
• 期刊: Journal of chromatography. A
• 作者: Stellwagen NC;Stellwagen E
• 通讯作者: Stellwagen E

Effect of magnesium ions and temperature on the sequence-dependent curvature of DNA restriction fragments.

镁离子和温度对 DNA 限制性片段的序列依赖性曲率的影响。

• DOI: 10.1088/0953-8984/22/49/494110
• 发表时间: 2010
• 期刊: Journal of physics. Condensed matter : an Institute of Physics journal
• 作者: Stellwagen,NancyC;Lu,Yongjun
• 通讯作者: Lu,Yongjun

Flanking A·T basepairs destabilize the B(∗) conformation of DNA A-tracts.

侧翼 A·T 碱基对会破坏 DNA A 束的 B(→) 构象的稳定性。

• DOI: 10.1016/j.bpj.2015.01.044
• 发表时间: 2015
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Stellwagen,Earle;Dong,Qian;Stellwagen,NancyC
• 通讯作者: Stellwagen,NancyC

Electrophoretic Mobilities of the Charge Variants of DNA and Other Polyelectrolytes: Similarities, Differences, and Comparison with Theory.

DNA 和其他聚电解质的电荷变体的电泳迁移率：相似点、差异以及与理论的比较。

• DOI: 10.1021/acs.jpcb.6b10599
• 发表时间: 2017
• 期刊: The journal of physical chemistry. B
• 作者: Stellwagen,NancyC

Curved DNA molecules migrate anomalously slowly in free solution.

• DOI: 10.1093/nar/gki748
• 发表时间: 2005
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Stellwagen E;Lu Y;Stellwagen NC
• 通讯作者: Stellwagen NC