MEMBRANE PROTEIN STRUCTURE : USE OF LIPIDIC MESOPHASES

描述：（改编自申请）此修订后的申请旨在 将Caffrey博士在脂质方面的专业知识应用于立方体脂质的合理设计， 用于膜蛋白结晶的阶段。虽然一些 膜蛋白，特别是细菌视紫红质（BR），已成功地 从脂质立方中间相结晶，还没有仔细研究 中间相的最佳状态，以及中间相和中间相之间的边界的细节。 生长晶体和体相。相反，只有经验程序 用于获得晶体。为了补救这一点，卡夫瑞博士建议延长他的 单酰基甘油的相图库，其形成立方中间相， 并研究了相图与最佳反应条件之间的关系 BR和细菌光反应中心RC的结晶。结果 这些研究中，连同晶体/脂质的详细分析， 界面将被用于尝试合理的结晶， 来自衣原体的先前未结晶的光系统II。

Description: (Adapted from the application) This revised application aims to apply Dr. Caffrey's expertise in lipids to the rational design of cubic lipid phases for the crystallization of membrane proteins. Though a number of membrane proteins, notably bacteriorhodopsin (BR), have been successfully crystallized from lipid cubic mesophases, there has been no careful study of the optimum state of the mesophase, and of the details of the boundary between the growing crystal and the bulk phase. Instead only empirical procedures are used to obtain crystals. To remedy this, Dr. Caffrey proposes to extend his library of phase diagrams for monoacylglycerols, which form cubic mesophases, and to study the relationship between the phase diagrams and the optimum crystallization of BR and of a bacterial photoreaction center, RC. The results of these studies, together with an analysis in detail of the crystal/lipid interface will then be used in attempts at rational crystallization of the previously uncrystallized photosystem II from Chlamydomonas.