FREE SOLUTION MOBILITY AS A PROBE OF DNA ELECTROSTATICS

描述：此修订后的新申请寻求资金，以表征 DNA静电特性的自由溶液迁移率观察 一系列DNA分子。第一个目标是衡量网络的效果 电荷对DNA在电场中迁移的影响。二是 研究DNA上有效电荷的序列依赖性 分子，后续的初步工作表明，这是情况。 结合FRET分析，提出的实验将挑战 在DNA小沟中结合单价互补物导致 静电崩溃并导致朝向小沟弯曲。进一步 这项工作需要检查DNA和常用的DNA之间的相互作用。 电泳实验中的缓冲液。

DESCRIPTION: This amended new application seeks funding to characterize features of DNA electrostatics by observation of the free solution mobilities of a number of DNA molecules. The first goal is to measure the effect of net charge on the migration of DNA in an electric field. The second is to investigate the sequence dependence of the effective charge on the DNA molecule, following up on preliminary work indicating that this is the case. Combined with FRET analysis, the experiments proposed will challenge the hypothesis that monovalent counterion binding in the minor groove of DNA causes electrostatic collapse and leads to bending towards the minor groove. Further work entails an examination of the interaction between DNA and commonly used buffers in electrophoresis experiments.