Training Program in Academic Nephrology

描述(由申请人提供)： 拟议的培训计划旨在继续为以前获得医学博士和/或博士学位的个人提供学术肾病方面的多学科研究生研究培训。被该计划录取的个人将花费三年或更长时间进行基本上全职的实验室研究。该计划将通过定期安排的一系列讲座和研讨会来丰富；还鼓励参与者在哈佛大学注册一些正式的研究生课程。我们相信，拟议计划中最重要的组成部分是学员在申请机构内紧张而几乎不受干扰地参与正在进行的研究项目，并由教员提供密切和持续的监督，教员本身也是NIH支持的研究项目的接受者和主要研究人员。重点将放在基础肾病和应用肾病的整合上。基础研究的培训机会领域将包括跨越肾上皮和内皮细胞的溶质和液体交换的生理、细胞和分子生物学基础，血管活性激素的生理学和药理学，肾脏疾病中的免疫和非免疫机制，以及移植的免疫生物学。这些基本方法也将尽可能应用于临床相关的研究领域，包括建立肾脏功能紊乱的动物模型；广泛的免疫学技术，以更好地确定T细胞对细胞表面抗原的识别，以及随后导致炎症免疫反应或耐受的基因激活系统；动物模型和人类移植受者移植排斥反应中效应机制的性质和控制。所代表的技术包括蛋白质和核酸生物化学、分子生物学、细胞和组织培养、免疫细胞化学、膜分离、电生理学、显微穿刺术和整个动物程序。

DESCRIPTION (provided by applicant): The proposed training program seeks to continue to provide multidisciplinary postgraduate research training in academic nephrology to individuals who have previously earned M.D., and/or Ph.D. degrees. Individuals accepted into this program will spend three or more years in essentially full-time laboratory research. The program will be enriched with a regularly scheduled series of lectures and seminars; participants are also encouraged to enroll in some formal postgraduate course work within Harvard University. It is our belief that the most important component of the proposed program is the intense and virtually undisturbed involvement of trainees in ongoing research projects within the applicant institution, with close and continuous supervision provided by faculty preceptors who are themselves the recipients and principal investigators of NIH supported research programs. Emphasis will be placed on integration of basic and applied nephrology. The domain of training opportunities in basic research will include the physiological, cellular and molecular biological bases of solute and fluid exchanges across renal epithelia and endothelia, physiology and pharmacology of vasoactive hormones, the immunological and non-immunological mechanisms operating in renal diseases, and the immunobiology of transplantation. These basic approaches will also be applied, wherever possible, to clinically relevant areas of investigation, including the creation of animal models of deranged functions of the kidney; extensive of immunological techniques to better define T cell recognition of cell surface antigens, and subsequent gene activation systems that lead to inflammatory immune responses, or to tolerance; the nature and control of effector mechanisms in graft rejection in animal models and in human transplant recipients. Techniques represented include protein and nucleic acid biochemistry, molecular biology, cell and tissue culture, immunocytochemistry, membrane fractionation, electrophysiology, micropuncture, and whole animal procedures.