All-in-One Biochip for Infectious Diseases Screening

描述(申请人提供)：在美国，每三秒就有人需要献血，这相当于每天大约使用4万品脱的血液。目前的血液筛查实验室测试非常耗时，必须对所有传染病“连续”进行。快速现场识别患有血液传播疾病的潜在献血者，将有助于消除不必要的采血、处理、储存、运输成本，并有助于防止进一步感染。该项目将开发一种一体化的传染病芯片(IDChip)技术，可以快速、灵敏地“同时”检测和识别多种(约10种)血液传播疾病。这款一体机IDChip包含了所有所需的试剂，因此从手指棒中加载非常小的体积是唯一需要手动参与的操作。这种一次性IDChip利用微制造和集成微流控技术，在圆盘封装中提供超灵敏的生化自动化。IDChip系统紧凑、便携，将有助于确保国家的血液供应不受传染病的影响。第一阶段的工作将侧重于IDChip平台的设计和制造、压力驱动微流体的构建、系统的集成、生物检测(乙肝表面抗原、抗-丙型肝炎病毒、抗甲型肝炎病毒、HIV-1)的性能，以及系统性能的评估。

DESCRIPTION (provided by applicant): Every three seconds someone in the United States requires donated blood, which correlates to approximately 40,000 pints of blood used each day. Current laboratory tests for blood screening are time consuming and must be performed "consecutively" for all infectious diseases. Rapid onsite identification of potential donors with blood borne diseases, will help to eliminate needless blood drawn, handling, storage, transportation costs, and will help to prevent further infections. This project will develop an all-in-one infectious diseases chip (IDChip) technology that can rapidly, sensitively, and "simultaneously" detect and identify multiple (approximately 10) blood transmissible diseases. This all-in-one IDChip contains all required reagents, so loading a very small volume from a finger-stick is the only manual involvement required. This disposable IDChip utilizes microfabrication and integrated microfluidic technologies to provide ultra-sensitive biochemistry automation in a disc package. The IDChip system is compact, portable, and will help to ensure that the nation's blood supply is free from infectious diseases. Phase I work will focus on design and fabrication of the IDChip platform, construction of pressure-driven microfluidics, integration of the system, performance of bioassays (HBsAg, anti-HCV, anti-HAV, HIV-1), and evaluation of the system's performance.