Quantitative Real Time PCR Instrumentation

加州大学旧金山分校的健康科学校区是世界上最重要的生物医学研究中心之一。它的医学院、药房、牙科和护理学院雇用了数百名杰出的研究人员，他们在获得NIH奖项方面有着卓越的记录。加州大学旧金山分校拥有最先进的生物技术核心设施——生物分子资源中心（BRC）。BRC自1985年以来一直服务于UCSF校园，并满足研究人员在DNA和肽测序，合成，分析和纯化方面的需求。它占地1900平方米。1英尺的实验室/办公空间。去年，大约有225名主要研究人员使用了该设施。大约190个pi测序了25500个DNA模板，105个pi合成了大约3000个寡核苷酸，超过30个pi合成了110个肽，大约20个pi测序了大约79个蛋白质。该设施由监督员委员会监督。Kathryn Ivanetich博士是BRC的董事，负责监督BRC的日常科学、财务和行政方面的工作。申请资金用于应用生物系统7900 TaqMan定量仪器与Zymark Twister自动化配件。该仪器被要求为UCSF的研究人员提供定量实时PCR作为一项新的BRC服务，并满足校园对这项技术的大量需求。这一请求得到了14名UCSF首席研究员的支持。这些pi代表了加州大学旧金山分校的14个院系，包括生物化学和生物物理学、医学、解剖学、微生物学和免疫学、内分泌学、神经外科、儿科和口腔医学，以及心血管研究所。所请求的工具将是一个真正的共享资源。没有一个单独的调查人员、调查小组或部门能主导它的使用。

The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators needs in DNA and peptide sequencing, synthesis, analysis and purification. It is housed in 1,900 sq. ft. of laboratory/office space. Approximately 225 principal investigators have used the facility in the last year. Approximately 190 PIs have had 25,500 DNA templates sequenced, 105 PIs had ca. 3,000 oligonucleotides synthesized, over 30 PIs have had 110 peptides synthesized, and approximately 20 PIs had approximately 79 proteins sequenced. The facility is overseen by a Board of Overseers. Dr. Kathryn Ivanetich is the Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 7900 TaqMan quantitative instrument with a Zymark Twister automation accessory. The instrument is requested to enable the provision of quantitative real time PCR as a new BRC service to UCSF investigators, and to meet the considerable on campus demand for this technology. This request is supported by 14 UCSF Principal Investigators. These PIs represent 14UCSF departments, including Biochemistry and Biophysics, Medicine, Anatomy, Microbiology and Immunology, Endocrinology, Neurological Surgery, Pediatrics, and Stomatology, plus the Cardiovascular Research Institute. The requested instrumentation would be a truly shared resource. No single investigator, group of investigators or department would dominate its use.