Request for Leica TCS SP2 Spectral Confocal Microscope

描述（由申请人提供）：我们要求资金用于购买 Leica光谱共聚焦显微镜（Leica TCS SP2 UV）。我们目前的BlO-RAD 600 显微镜是10岁，不再适合目前的标准， research.布兰迪斯大学目前没有任何其他共焦 成像设备。事实上，最先进的共焦技术是绝对必要的 送到国家卫生研究院资助用户群的研究。它也将使其他 布兰迪斯的生命科学研究人员对生物材料成像感兴趣。 在用户组的10名调查人员中，有7名是当前用户， 另外三位是研究人员，他们的研究现在已经准备好采取 共焦成像的优点。项目包括： 形成，突触可塑性，神经调节，神经递质调节 释放，神经元多样性发育的潜在机制，定位 生物节律相关的蛋白质，性行为的定位 相关蛋白质，RNA加工和运输机制，以及 细胞内的细胞器运输。 Leica TCS 2 SP2仪器将安装在成像设备中， 我们的旧共焦系统就在这里因为我们有一个共焦设备， 10年来，运行和维护机制已经到位。的 成像设施由一名“成像专家”管理，该专家还负责监督 共聚焦显微镜的日常操作，包括新用户的培训 仪器的维护。布兰代斯大学与生物学 部门致力于维护这一设施。除了用户 小组，共聚焦设施将提供给所有生命科学研究人员 在布兰迪斯谁有需要的共焦成像的时间可用的基础上。

DESCRIPTION (provided by applicant): We request funds for the purchase of a Leica Spectral Confocal Microscope (Leica TCS SP2 UV). Our current BlO-RAD 600 microsocope is 10 years old and no longer adequate for current standards of research. Brandeis University does not currently possess any other confocal imaging facility. In fact a state of the art confocal is absolutely essential to the N.I.H. funded research of the user group. It will also benefit other life science researchers at Brandeis interested in imaging biological material. Of the ten investigators in the user group, seven are current users and the other three are investigators whose research is now well poised to take advantage of confocal imaging. Projects include: mechanisms of synaps formation, synaptic plasticity, neuromodulation, regulation of neurotransmitter release, mechanisms underlying development of neuronal diversity, localization of biological rhythm associated proteins, localization of sexual behavior associated proteins, mechanisms in RNA processing and transport, and mechanisms of intracellular organelle transport. The Leica TCS2 SP2 instrument would be housed in the Imaging Facility that currently houses our old confocal. Because we have had a confocal facility for 10 years, mechanisms of operation and maintenance are already in place. The Imaging facility is managed by an "Imaging Specialist" who also oversees the day to day operation of the confocal microsope including training of new users and maintenance of the instrument. Brandeis University and the Biology Department are committed to maintain this facility. In addition to the user group, the confocal facility will be available to all life science researchers at Brandeis who have a need for confocal imaging on a time-available basis.