COPAS SELECT DROSOPHILA EMBRYO AND LARVA SORTER

该提案是为果蝇购买复杂对象参数分析仪和分类器（COPAS）。该仪器能够分选大量的转基因果蝇胚胎。这是最近可用的技术，其使得能够进行各种功能基因组学研究，包括1）使用突变动物的基因表达微阵列实验。2)使用突变果蝇胚胎的染色质-DNA共免疫沉淀实验，和3）基于转基因动物中GFP荧光的激活或失活的新型遗传筛选。我们建议购买该仪器，供耶鲁大学研究人员立即使用。我们意识到，这种新的仪器将允许各种各样的基因组学应用在果蝇，我们预计，在第一年的操作，最初的用户组将要求大部分可用的时间在仪器上。然而，由于大多数新仪器都允许进行以前技术上非常困难或不可能的研究，因此我们预计耶鲁大学的用户群将增长。从一开始，我们将采用一个使用跟踪和管理系统，以最大限度地利用这一工具，以当地社区的研究人员谁使用果蝇在他们的研究。

This proposal is for the purchase of a Complex Object Parametric Analyzer and Sorter (COPAS) for Drosophila. This instrument is capable of sorting large quantities of genetically modified Drosophila embryos. This is a recently available technology that enables a variety of functional genomics investigations including 1) Gene expression microarray experiments using mutant animals. 2) Chromatin-DNA co- immunoprecipitation experiments using mutant Drosophila embryos, and 3) Novel genetic screens based on activation or deactivation of GFP fluorescence in transgenic animals. We propose to purchase this instrument for these immediate uses by Yale Investigators. We realize that this new instrumentation will allow for a wide variety of genomics applications in Drosophila, and we expect that for the first year of operation, the initial user group will demand most of the available time on the instrument. However, as it is the case for most new instrumentation that allows research that was previously exceedingly technically difficult or impossible, we expect that the user group will grow at Yale. From the onset, we will employ a system of usage tracking and management in order to maximize the utility of this instrument to the local community of investigators who use Drosophila in their research.