NMR Console Upgrade and Cryoprobe

我们申请资金，以便更换位于耶鲁大学分子生物物理和生物化学贝斯中心的瓦里安统一500 MHz核磁共振光谱仪的控制台，并为该光谱仪购买一个冷冻探头。所要求的瓦里安·伊诺瓦游戏机将取代大约在1990年建造的Unity游戏机，并于1995年被二手耶鲁大学购买。Inova游戏机拥有更强大的计算机界面，需要更少的重启，并且比Unity游戏机更高效地执行脉冲、功率水平、相位变化和频率变化。这一升级应会显著改进脉冲序列的实现。此外，Inova游戏机与VARIAN最新软件SPINCAD兼容，SPINCAD不能在Unity或Unity Plus系统上运行。因此，此次升级应可确保我们的500 MHz光谱仪在未来数年内继续使用。最后但并非最不重要的一点是，更换有问题的控制台将减少仪器维修损失的时间，这一点已经变得相当重要，因为Unity控制台的使用寿命即将结束。所要求的冷冻探测器最近由瓦里安开发。它的主要吸引力在于相对于室温探头的高灵敏度。配备低温探头的500 MHz光谱仪的灵敏度应该是耶鲁800 MHz光谱仪上目前最好的室温探头观测到的灵敏度的两倍。有几个正在考虑的结构生物学项目需要这种敏感性，因为当使用标准探针时，感兴趣的分子聚集在所需的浓度。

We request funding so that the console of the Varian Unity 500 MHz NMR spectrometer housed in the Yale Department of Molecular Biophysics and Biochemistry Bass Center can be replaced, and a cryoprobe purchased for that spectrometer. The Varian Inova console requested will replace a Unity console that was built ca. 1990, and was purchased by Yale, second hand, in 1995. Inova consoles have a more robust computer interface that requires fewer reboots, and that executes pulses, power level, phase changes and frequency changes more efficiently than Unity consoles. This upgrade should result in significantly improved implementations of pulse sequences. In addition, Inova consoles are compatible with Varian newest software, SPINCAD, which do3es not run on Unity or Unity plus systems. Thus this upgrade should ensure the usefulness of our 500 MHZ spectrometer for years to come. Last, but not least, replacement of the console in question will reduce the amount of instrument time lost for repair, which has become considerable because the Unity console is reaching the end of its useful life. The cryoprobe requested has recently been developed by Varian. Its major attraction is its high sensitivity, relative to room temperature probes. The sensitivity of the 500 MHz spectrometer equipped with a cryoprobe should be double the sensitivity observed with the best room temperature probe now available on the Yale 800 MHz spectrometer. There are several structural biology projects under consideration that demand this sensitivity because the molecules of interest aggregate at the concentrations required when standard probes are used.