Acquisition of High Speed Cell Sorter

描述(由申请人提供)：在本建议书中，我们寻求资金以 购买一台高速三激光细胞分选机。分拣机将安装在 现有的流式细胞术核心设施位于Skirball研究所和 由卡普兰综合癌症中心运营。它将取代单一的 激光FACStar Plus细胞分选机尚未投入使用 被拆除了。 新的分拣机将具有分拣速度约为 每秒20,000-25,000个事件，并对最多四个不同的群体进行排序， 这将使研究存在于非常 原始样本中的低频率。三种激光的组合 将允许使用当前可用的有效多色分类和分析 还将为纽约大学医学院的快速 不断增加的新的流式细胞仪应用清单。新版本的特点 Sorter将通过以下方式提高实验设计的灵活性 免疫学家，他们传统上使用细胞分选，还将 促进细胞和发育生物学家日益增长的需求以及 神经生物学家对稀有细胞群进行分类以研究基因靶标 干细胞和祖细胞的活性和发育潜力。 新的细胞分类器将提高正在进行的项目的有效性 在医学院的12名研究人员的实验室里进行的。 所有这些调查人员，除了一名新的初级教员 成员，都得到了NIH资金的支持。此外，该仪器将被 可供整个纽约大学医学中心社区使用，并将增强 许多其他小组的研究。它的可用性将增强我们的能力 吸引有经验的免疫学家和癌症生物学家在我们目前的 招聘高级和初级教师。我们相信，可获得的 我们校园里的这台强大的机器将引发新的令人兴奋的研究项目 来自癌症、免疫学和医学领域的众多科学家 动物发育。

DESCRIPTION (provided by applicant): In this proposal, we seek funds to purchase a high speed, three-laser cell sorter. The sorter will be installed in the existing Flow-Cytometry core facility located at the Skirball Institute and operated by the Kaplan Comprehensive Cancer Center. It will replace a single laser FACStar Plus cell sorter that has not been operational and has been dismantled. The new sorter will have the capability to sort at speeds of about 20,000-25,000 events per second, and to sort up to four different populations, which will make possible the study of sub-populations of cells present at very low frequencies in the original samples. The combination of the three lasers will permit effective multicolor sorting and analysis using currently available fluorochromes and will also prepare NYU School of Medicine for the rapidly increasing list of new flow-cytometry applications. The features of the new sorter will allow increased flexibility in experimental design by immunologists, who have traditionally used cell sorting, and will also facilitate the growing needs of cell and developmental biologists as well as neurobiologists to sort rare populations of cells to study targets of gene activity and the developmental potential of stem cells and progenitor cells. The new cell sorter will improve the effectiveness of ongoing projects being carried out in laboratories of twelve investigators at the School of Medicine. All of these investigators, with the exception of one new junior faculty member, are supported by NIH funding. In addition, the instrument will be available to the entire NYU Medical Center community, and will enhance the research of many other groups. Its availability will enhance our ability to attract accomplished immunologists and cancer biologists in our current recruitment of senior and junior faculty. We believe that the availability of this powerful machine on our campus will elicit new exciting research projects from a variety of scientists working in the fields of cancer, immunology, and animal development.