Collagen Based Bioactive Electrospun Scaffolds for Bone Tissue Engineering

大多数天然组织都含有大量的胶原蛋白，这是一种天然聚合物。骨由I型胶原蛋白组成，含有大约40vol%的骨矿物质，是一种羟基磷灰石(Ca10(PO4)6(OH)2)。该项目的目的是优化基于双芯电纺的生物活性多孔支架的生产。这将被用来覆盖每一种缓慢降解的延性聚合物的纤维，外层更具生物活性，吸引细胞加速骨形成。内部纤维核心将是聚己内酯，外层临床I型胶原将在体外矿化或制造含有HA。天然胶原为组织提供了细胞外基质(ECM)的骨架。胶原蛋白比大多数聚合物具有更高的生物活性和生物相容性，但供应系统复杂，以确保临床级别的材料，并需要仔细处理，以确保所生产的材料/设备的受控降解，因为该项目将由胶原解决方案有限公司提供。此外，多肽已被用于模拟细胞外基质的自然组织纤维结构。人工合成的离子自组装寡肽(ISAP)具有通过特异性配体-受体相互作用来驱动不同细胞反应的能力。在这项研究中，我们将使用一种新型的ISAP对支架表面进行功能化处理。复合材料的优化将基于机械性能，主要是强度和延展性，然后是生物兼容性和生物活性的评估，并进一步优化。

Most natural tissues contain substantial amounts of collagen, a natural polymer. Bone consists of Type I collagen reinforced with approximately 40vol% bone mineral, a version of hydroxyapatite (Ca10(PO4)6(OH)2). The aim of this project is to optimise the production of a bioactive, porous scaffold based on dual core electrospinning. This will be used to coat each fibre of a slowly degrading ductile polymer, with a more bioactive outer layer, attracting cells to accelerate bone formation. The inner fibre core will be polycaprolactone, the outer layer clinical grade Type I collagen to be either mineralised in vitro or manufactured containing HA. Natural collagen provides a framework of extracellular matrix (ECM) for tissues. Collagen is substantially more bioactive and biocompatible than most polymers, but the supply system is complex to ensure clinical grade material and needs careful processing to ensure controlled degradation of the materials/device produced, for this project will be supplied by Collagen Solutions Ltd. Additionally, peptides have been employed to mimic natural tissue fibrous structures of the extra cellular matrix. Synthetic ionic self-assembly oligo-peptides (iSAP) have the ability to drive differential cell responses by specific ligand-receptor interaction. In this study we will functionalise the scaffold surfaces with a novel iSAP. Composite optimisation will be based on mechanical properties, principally strength and ductility, followed by assessment of biocompatibility and bioactivity with further optimisation.