XRAY DATA COLLECTION OF RNA POLYMERASE II & DNA CO CRYSTAL

RNA聚合酶II/DNA的共晶体最近已经获得， 衍射到5 E的双阳极X射线源。 考虑到 利用双阳极X射线从5E分辨率显著提高 在同步加速器光源上对聚合酶进行平行研究， 单独的共晶，预计将在同步加速器的作用下， 梁也是。 已经开发了一种低温浸泡程序， 使得从一个晶体收集数据是可行的。 我们现建议 收集聚合酶II/DNA的高分辨率X射线衍射数据 共晶及其重原子衍生物。 目标是 获得用于确定晶体的高质量衍射数据 RNA聚合酶II/DNA复合物结构。

Cocrystal of RNA polymerase II/DNA has been recently obtained with diffraction to 5 E on rotating-anode X-ray source. In view of the significant improvement from 5 E resolution with rotating-anode X-ray to 3 E at the synchrotron source in a parallel study of the polymerase alone, the cocrystal is expected to diffract to 3 E with synchrotron beam as well. A cryosoaking procedure has already been developed, making data collection from one crystal feasible. We now propose to collect high resolution X-ray diffraction data on polymerase II/DNA cocrystal as well as its heavy atom derivatives. The goal is to obtain high quality diffraction data for determining the crystal structure of RNA polymerase II/DNA complex.